Labshake search
Citations for GE Life Sciences :
351 - 400 of 943 citations for IL 17RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... cell culture supernatant was harvested and purified with a Protein A (GE Healthcare, Chicago, IL) column ...
-
bioRxiv - Biochemistry 2022Quote: ... followed by GST column purification using the ÄKTA start chromatography system (GE Healthcare, Chicago, IL). Cell lysate supernatant was added to the column containing nickel resin and it was recirculated across the column by a peristaltic pump overnight at 0.5 ml/min ...
-
bioRxiv - Biophysics 2022Quote: ... Fluorescence-labeled protein was visualized with a Typhoon trio fluorescence scanner (GE Healthcare, Chicago, IL) and relative protein quantities were determined using FIJI (Schindelin et al. ...
-
bioRxiv - Biophysics 2022Quote: ... the protein sample was loaded on an S75 gel-filtration column (GE Healthcare, Chicago, IL). Pure fractions were collected and dialyzed against 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2019Quote: ... and separated on a Superose 6 Increase column (GE Healthcare Life Sciences, Chicago, IL, USA) on an NGC Chromatography system (Bio-Rad Laboratories ...
-
bioRxiv - Genomics 2019Quote: ... and filter sterilizing through 0.2 μm syringe filters (Whatman GE Healthcare Life Sciences, Chicago, IL) into growth medium ...
-
bioRxiv - Plant Biology 2019Quote: ... The supernatant was filtered using a 0.45-μm polypropylene filter plate (GE Healthcare, Chicago, IL). Samples were separated by reversed-phase chromatography on an Ultimate 3000 HPLC (Dionex ...
-
bioRxiv - Biochemistry 2019Quote: ... and collected by a Frac-920 fraction collector (GE Healthcare Life Sciences, Chicago, IL, USA). 100 mg of commercial Enoxaparin Sodium Injection was injected onto the SEC column and separated by using 0.5 M ammonium bicarbonate eluent at a flow rate of 0.2 mL/min ...
-
bioRxiv - Biochemistry 2019Quote: ... Clear lysate was loaded on a 5 mL HisTrap HP column (GE Healthcare, Chicago, IL) pre-equilibrated with buffer A ...
-
bioRxiv - Immunology 2020Quote: ... Protein was column-purified on Nap-5 columns (GE healthcare, Chicago, IL; #17-0853-01) and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare, Chicago, IL, USA). The membranes incubated with the appropriate antibody in Tris buffered with Tween-20 (TTBS) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membranes were then imaged by ImageQuant LAS4000 luminescent Image Analyzer (GE Healthcare, Chicago, IL) following an incubation with ECL chemiluminescent substrate reagent kit (ThermoFisher ...
-
bioRxiv - Biochemistry 2021Quote: ... was purified by IMAC on a Ni2+-His-Trap column (GE Healthcare, Chicago, IL, USA) in buffer 0,1M K-phosphate pH 7,5 ...
-
bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... Cell lysate was loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA) equilibrated with Buffer A (300 mM NaCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
-
Interspecific introgression and natural selection in the evolution of Japanese apricot (Prunus mume)bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was extracted from young leaves using Nucleon PhytoPure (GE Healthcare, Chicago, IL, USA) and subjected to phenol/chloroform purification ...
-
bioRxiv - Immunology 2021Quote: ... bands were detected by adding substrate solution in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL). For detecting other tags and proteins ...
-
bioRxiv - Microbiology 2021Quote: ... Blots were imaged by Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare, Chicago, IL) and Chemidoc (Bio-rad) ...
-
bioRxiv - Microbiology 2021Quote: ... the dye values were measured using ImageQuant TL version 8 (GE Healthcare, Chicago, IL, USA) and Adobe Photoshop Elements were normalized to density values per mm2 of a worm’s projection area ...
-
bioRxiv - Immunology 2022Quote: ... and Fab fractions were further purified by cation exchange chromatography (MonoS, GE Healthcare, Chicago, IL). His-tagged wildtype RBD (BEI NR52309) ...
-
bioRxiv - Microbiology 2022Quote: ... which were visualized by exposure to an X-ray film (GE healthcare, Bloomington, IL, USA). The protein expression of TLRs (n = 5 pigs each group ...
-
bioRxiv - Cancer Biology 2022Quote: ... were added together with 40 μL of protein A-Sepharose (GE Healthcare, Chicago, IL, USA) to mixed cell lysates containing up to 20 mg of total labeled proteins and incubated at 4 °C for 4 h ...
-
bioRxiv - Immunology 2022Quote: ... and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Genetics 2023Quote: ... and purity and concentration were determined by a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). An RNA pool for each biological replicate was generated by mixing the samples extracted from the picked berries ...
-
bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
-
bioRxiv - Plant Biology 2023Quote: ... The secondary antibody was Donkey anti-rabbit IgG-HRP (NA934, GE Healthcare, Chicago, IL, USA), which was detected using ECL select (Cytiva) ...
-
bioRxiv - Cell Biology 2023Quote: Gel-filtration chromatography was performed with the AKTA purifier system (GE Healthcare, Chicago, IL, USA) controlled by the Unicorn 5.1 software ...
-
bioRxiv - Biochemistry 2023Quote: ... All gels were visualized on a Typhoon FLA 9500 imaging system (GE Healthcare, Chicago, IL) and quantified using Quantity One software (Bio-Rad Laboratories ...
-
bioRxiv - Plant Biology 2023Quote: ... The RNA yield was quantified using a NanoVue microplate reader (GE Healthcare, Chicago, IL, USA). The sample volume was adjusted to 2 µg/100 µL for subsequent RNA-seq analysis ...
-
bioRxiv - Plant Biology 2023Quote: ... and then analyzed by a Typhoon FLA 7000 image analyzer (GE Healthcare, Chicago, IL., USA) (Fukuhara et al. ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with either Glutathione Sepharose 4B (GE Healthcare, Chicago, IL, USA), Complete His-Tag Purification Resin (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with either Glutathione Sepharose 4B (GE Healthcare, Chicago, IL, USA) or Complete His-Tag Purification Resin (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the his-tagged FABP1 was purified using HisTrap HP affinity column (GE Healthcare, Chicago, IL), the tag was cleaved by thrombin and the cleaved protein was purified and buffer exchanged by gel filtration into 10 mM potassium phosphate pH 7.4 ...
-
bioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (goat anti-mouse and anti-rabbit) (GE HealthCare, Chicago, IL, USA) were used at dilutions of 1:20 000 and 1:10 000 ...
-
bioRxiv - Biochemistry 2024Quote: ... and detected using a high-sensitivity imaging system (LAS-4000, GE healthcare, Chicago, IL, USA).
-
bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
-
bioRxiv - Neuroscience 2020Quote: Binding kinetics of VNAR-Fcs was determined by surface plasmon resonance (SPR) using a Biacore T200 (GE Healthcare). A His-capture kit (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure (GE Healthcare Life Sciences) column according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
-
bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
-
bioRxiv - Developmental Biology 2021Quote: ... Chemiluminescence was detected by ECL Prime Western Blotting Detection Reagents (RPN2232, GE Healthcare, Chicago, IL, USA) using the Image Quant LAS 4000 mini (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Chemiluminescence was detected by ECL Prime Western Blotting Detection Reagents (RPN2232, GE Healthcare, Chicago, IL, USA) using the Image Quant LAS 4000 mini (GE Healthcare) ...
-
bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2021Quote: ... the cell pellets were resuspended in 10 mL of 40% Percoll gradient (GE Healthcare, Chicago, IL) for centrifugation at 700 g for 20 min at room temperature without brake ...
-
bioRxiv - Molecular Biology 2020Quote: ... RSFPs were isolated from the supernatant with the His SpinTrap kit (GE Healthcare, Chicago, IL, USA) and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius ...
-
bioRxiv - Molecular Biology 2020Quote: ... Mononuclear cells were enriched using a Ficoll gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, IL) for 25min at 2,000g at room temperature ...