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Citations for GE Life Sciences :
351 - 400 of 1572 citations for Caspase Recruitment Domain Family Member 17 CARD17 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded on a gravity chromatography column preloaded with glutathione-Sepharose 4B (17-0756-05, GE Healthcare) and incubated for 2 h at 4 °C with gentle rotation in batch mode ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Samples were further purified by ion exchange on a HiTrap Q column (GE Healthcare Life Sciences #17-1153-01) and eluted in 250 μl aliquots of 20/250 TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Immunology 2019Quote: PBMCs were isolated from whole human peripheral blood by Ficoll-Paque PLUS (17-1440-03, GE Healthcare, Pittsburgh, PA) density gradient centrifugation and cryopreserved prior to use ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended in approximately 2 mL of 100% Percoll solution (Cat. no. 17-0891-01, GE Healthcare), and layered carefully on top of 3 mL of 80% and 40% Percoll solution ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Which Peripheral blood after dilution were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The peripheral blood leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was centrifuged and leukocytes were further separated using 40% Percoll density gradient (GE Healthcare Cat# 17-0891-01) and centrifugation (1400 x g ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length GFAT-1 and the isomerase domain were further purified according to their size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Neuroscience 2020Quote: ... and hyperphosphorylated 2N4R tau produced in Sf9 cells to LRP1 or the VSP10 domain of SORL1 were assessed using a Biacore 3000 optical biosensor system (GE healthcare Life Sciences) essentially as described (M ...
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bioRxiv - Cell Biology 2020Quote: Binding of Wbox2 to SNX9 and AP2-β2-hinge+appendage domain was investigated by ITC using a MicroCal iTC200 (GE Healthcare Life Sciences). Measurements were performed in 20mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing the tagged domain were pooled and exchanged to the initial buffer containing 5 mM imidazole by using a PD10 desalting column (GE Healthcare Life Sciences). His-tagged TEV protease (0.1 mg/ml final concentration ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... Eluted Sox2 was refolded by changing to a zero-urea buffer using a desalting column (GE healthcare #17-1408-01). Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: Cells for single cell RNA-seq were prepared by density centrifugation using Ficoll-Paque media (GE Healthcare, 17-5446-02) followed by magnetic-activated cell sorting (MACS ...
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bioRxiv - Biochemistry 2020Quote: ... Medium was collected 48-60 h post infection and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) pre-equilibrated with Buffer A (150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cancer Biology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll®-Paque PLUS, GE Healthcare, 17-1440-03) from healthy donors ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were purified by a density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare Life Sciences, Cat# 17-1440-03) and stored in liquid nitrogen until further use.
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The medium from 1 L of culture was filtered using a 0.22-μm membrane and the filtrate was adjusted to contain 30 mM imidazole before incubating with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) beads for 2 h at 4 °C with mild agitation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from EDTA blood using density gradient separation in Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 (10/300) column (#17-5172-01) and Superdex 200 increase 3.2/300 column (#28-9909-46) from GE Healthcare; Trans-blot turbo transfer pack (#1704158 ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were isolated and purified by Percoll density gradient (72%, 63%, 54%, and 45%) (GE Healthcare; Cat. 17-5445-01). Isolated neutrophils were resuspended in RPMI 1640 (Corning ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtrated through 0.45 µm filter and loaded into HisTrap HP column (GE Healthcare, Cat#:17-5248-02). The collected fractions were concentrated by Amicon® Ultra-15 Centrifugal Filter Units (EMD Millipore ...
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bioRxiv - Immunology 2023Quote: ... resuspended in HBSS and 20mM HEPES and loaded onto a 62% (v/v) Percoll gradient (GE Healthcare #17-0891-01) in HBSS and 20mM HEPES ...
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bioRxiv - Microbiology 2022Quote: Neutrophils were purified by centrifugation for 30 min at 1,300xg on a discontinuous Percoll gradient (GE Healthcare #17-0891-01) consisting of 62% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutants were expressed in BL21 as a standard procedure and then purified with glutathione-sepharose (GE Healthcare, 17-0756-01) as previously described [13] ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Genomics 2023Quote: ... and lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Immunology 2024Quote: Whole blood was collected in heparin tubes and PBMCs were isolated by Ficoll-Paque (GE Healthcare, Cat: 17-1440-03) gradient centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction solution was sequentially precipitated using protein A sepharose beads and Glutathione Sepharose beads (#17-0756-01, GE Healthcare). The resulting supernatant was stored at −20 °C until it was analyzed for the surface plasmon resonance (SPR ...