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Citations for GE Life Sciences :
351 - 400 of 3572 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The concentrated protein sample was centrifuged at 10,000 × g and 4°C for 10 min before the supernatant was loaded onto a lysis buffer equilibrated Superose 6 increase 10/300 GL column (GE Healthcare), connected to a 1260 Bioinert Infinity LC system and equipped with a multi-angle light scattering detector (miniDawn 3141MD3 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were eluted with HBS containing 200 mM imidazole and further purified using size-exclusion chromatography (Superdex 200 Increase 10/300 or Superose 6 Increase 10/300 columns; GE Healthcare) in 10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: Enzymatic degradations were monitored by analytical gel permeation chromatography using Superdex S200 10/300 and Superdex peptide 10/300 (GE Healthcare) columns mounted in series and connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... and followed by size-exclusion chromatography (SEC) using a superdex 200 Increased 10/300 chromatography column on an AKTA Purifier 10 FPLC system (GE Healthcare) with buffer containing 20 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ligation mix was diluted to 50µl and used 0.583:1 volume of home-brewed SPRI beads (SPRI binding buffer: 2.5M NaCL, 20mM PEG 8000, 10mM Tric-HCL, 1mM EDTA, oh=8, 1mL TE-washed SpeedMag beads GE Healthcare, 65152105050250 per 100mL buffer) for purification ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL or Superose 6 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples at concentrations >10 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were concentrated with 30 kDa cutoff concentrators (Amicon) to ∼10 mg/ml and loaded onto a S200 Increase 10/300 GL (GE Life Sciences) in SEC Buffer [20mM Tris ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% FBS (HyClone, GE Healthcare Life Science), penicillin (100 units/ml ...
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bioRxiv - Genetics 2021Quote: ... 10 ng (0.02 U) poly(dI-dC) (GE healthcare) and approximately 0.5 ng of 32P-labeled oligonucleotides were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and Superdex 200 10/300 GL column (GE Healthcare) was equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 percent heat-inactivated FBS (GE Healthcare Bio-Sciences), and IL-2 at 5 ng/ml (PeproTech) ...
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bioRxiv - Neuroscience 2021Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone) for 3.5 hours allowing the dextran to be delivered to the lysosomes (chase pulse) ...
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bioRxiv - Neuroscience 2021Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone). Primary microglia were plated and allowed to rest 24 hours prior to the experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Superdex75 10/300 GL column (GE Healthcare) in buffer containing 30 mM HEPES pH 7.9 ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Superdex75 10/300 GL column (GE Healthcare) in a buffer containing 30 mM HEPES pH 7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% bovine growth serum (GE Life Sciences), 10 units/mL penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... and 10% fetal bovine serum (FBS, GE Healthcare Hyclone) to ensure the presence of the complement molecules ...
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bioRxiv - Biochemistry 2019Quote: ... see below (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... Desalting was conducted using PD-10 column (GE healthcare) following manufacture’s protocol.
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bioRxiv - Plant Biology 2019Quote: ... pH 7.9 by PD-10 Desalting Columns (GE Healthcare). The 6xHis-Tag was removed by overnight digestion with thrombin protease ...
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bioRxiv - Biochemistry 2019Quote: ... using a PD-10 desalting column (GE Life Sciences). Protein was loaded onto a MonoQ anion exchange column (GE Life Sciences ...
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bioRxiv - Biochemistry 2019Quote: ... A Superdex 75 10/300 GL column (GE Healthcare) was both equilibrated and run in 20 mM Hepes ...
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bioRxiv - Immunology 2019Quote: ... containing 10% fetal bovine serum (GE Healthcare, Pittsburgh, PA); 50 ng/ml Granulocyte-macrophage colony-stimulating factor (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... both supplemented with 10% bovine growth serum (GE Healthcare) and 1% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... or a Superdex75 increase 10/300 column (GE Healthcare) using an AKTA pure (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... connected to an ÄKTA Purifier 10 system (GE Healthcare) and eluted with 1 CV ClpP-GF buffer ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% HyClone cosmic calf serum (GE Healthcare). Each transfection was with 15 µg total DNA at a 50%/37.5%/12.5% ratio of expression vector/pSPAX2/pMD2.G ...
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bioRxiv - Biochemistry 2021Quote: ... a Superdex 200 Increase 10/300 column (GE Healthcare) operated at a 0.8 ml/min flow rate was used (100 μl loading) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were applied to PD-10 columns (GE Healthcare) to remove residual Zn2+ ...
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bioRxiv - Biochemistry 2022Quote: Using an ÄKTApurifier UPC 10 FPLC system (GE Healthcare), lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection ...
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bioRxiv - Microbiology 2022Quote: ... Superdex 200 Increase 10/300 GL column (GE Healthcare) was pre-equilibrated overnight at 0.1 ml/minute flow ratewith buffer E (20 mM HEPES pH 8.0 ...