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Citations for GE Life Sciences :
351 - 400 of 3342 citations for 6 methyl 3 propan 2 ylcyclohex 2 en 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended in approximately 2 mL of 100% Percoll solution (Cat. no. 17-0891-01, GE Healthcare), and layered carefully on top of 3 mL of 80% and 40% Percoll solution ...
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bioRxiv - Microbiology 2020Quote: ... 2 mM EDTA) for 90 minutes at 4° Celsius and then scanned and examined by Typhoon trio+ (GE Healthcare) scanner using Cy5 channel.
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bioRxiv - Microbiology 2020Quote: ... Perkin Elmer with 100 Ci/ml for 2 hrs and immunoprecipitation of Fcγ fragments using CNBr-Sepharose (GE Healthcare) was performed as described previously (Sprague et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... in Can Get Signal Solution 2 (Toyobo) and visualized with ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232). Chemiluminescent signals were detected using a LAS-3000 Imaging System (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cultures grown in liquid conditions were harvested using a vacuum filtration with Whatman #2 paper (GE Healthcare, 47 mm). After 24 h of treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a Hyperfilm™ high performance autoradiography film (GE Healthcare) at −80 °C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM DTT.) Cascade was further purified by size exclusion chromatography using a Superose 6 increase column (GE healthcare) in SEC buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: ... followed by purification via Superose 6 (GE Healthcare). The proteins were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Immunology 2021Quote: ... or pH 6-11 (GE Healthcare, Uppsala, Sweden). With IPG strip reduction ...
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bioRxiv - Neuroscience 2019Quote: ... 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% sarkosyl (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... or IgG Sepharose (6 Fast Flow, GE Healthcare) (500 μl per 500 ml culture) ...
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bioRxiv - Microbiology 2021Quote: ... Ni Sepharose 6 Fast flow resin (GE Healthcare) affinity exclusion chromatography was performed to obtain the tagless protein (containing the tag C-terminal sequence ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA flux 6 system (GE Healthcare, USA). The system and cassette were sanitized with 1.0 N NaOH before and after diafiltration ...
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bioRxiv - Immunology 2023Quote: ... Superose 6 Increase 10/300 GL (GE Healthcare) in a mobile phase of PBS containing 300 parts per million of sodium azide and at a flow rate of 0.75 mL/min (Dionex Ultimate 3000 pump ...
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bioRxiv - Biochemistry 2023Quote: ... or Superpose 6 10/300 GL (GE Healthcare) size exclusion column ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-mm-diameter Whatman paper discs (GE Healthcare) were placed on top of the plate and wetted with 5 μl of a 10 mM TcmX solution in DMSO or 300 μg of erythromycin diluted in 10 μl of ethanol ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... filter and subjected to Superose 6 (GE Healthcare) Increase column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2024Quote: ... IMAC Sepharose 6 Fast Flow beads (GE Healthcare) was added into (Ribosome purification ...
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bioRxiv - Molecular Biology 2019Quote: ... concentrated to a volume of 2 ml and applied onto a S75 16/600 pg size exclusion column (GE Healthcare), equilibrated with 10 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 200 mM-300 mM NaCl in 20 mM HEPES (pH 7.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected and incubated with 2 column volume (for 20 mL supernatant) of Gluthatione Sepharose 4B (GE Healthcare) for 1h30 on a rotating wheel ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.Coli Rosetta 2 (DE3) and purified by affinity chromatography over a Sepharose 4B GSH affinity column (GE healthcare) followed by gel filtration over a Hiload Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 150 mMà350 mM NaCl in 20 mM Immidazole (pH 8.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Cell Biology 2019Quote: U-2 OS FUCCI cells were grown on a 96-well glass bottom plates (Whatman, Cat# 7716-2370, GE Healthcare, UK ...
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bioRxiv - Immunology 2020Quote: ... 7.5×104 congenically distinct cells of each sorted memory population were adoptively transferred and tumor-bearing mice were subsequently immunized with 10 μg of GP33-41 and 2 μg of poly(I:C) (GE Healthcare) s.c ...
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bioRxiv - Genetics 2019Quote: Germ cells and embryos were mounted on 2% agarose pads and imaged live using a DeltaVision Elite microscope (Applied Precision) with a 60x Silicon oil objective lens ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...