Labshake search
Citations for GE Life Sciences :
351 - 400 of 2071 citations for 5 Methoxypyridine 2 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The sample was injected in S200 5/150 column (GE Healthcare) pre-equilibrated with KMNZ reconstruction buffer and the peak fraction was taken for structural analysis.
-
bioRxiv - Bioengineering 2024Quote: ... and loaded onto a HisTrap HP 5 ml column (GE Healthcare) at 4 °C using an ÄKTA Pure 25 M (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA HisTrap columns (GE Healthcare, Chicago, IL, USA) were used ...
-
bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatant was applied to HisTrap HP 5 ml column (GE Healthcare) pre-equilibrated in 25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) as previously described3 except that an optimized buffer gradient was employed consisting of a first wash step of 5 column volumes (CV ...
-
bioRxiv - Bioengineering 2023Quote: ... with Nickel affinity chromatography (5 mL HisTrap HP column, GE Healthcare), anion ion exchange chromatography (5 mL HiTrap Q FF column ...
-
bioRxiv - Bioengineering 2023Quote: ... Affinity chromatography utilizing a HisTrap HP 5 mL column (GE Healthcare) was then employed to purify the protein in binding buffer consisting of 50 mM NaPO4 (pH 7.4) ...
-
bioRxiv - Cell Biology 2022Quote: ... before loading onto a 5 ml HisTrap HP column (GE Healthcare). The protein was eluted in 300 mM imidazole ...
-
bioRxiv - Bioengineering 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
-
bioRxiv - Biochemistry 2024Quote: ... and 5% glycerol using an AKTA Start FPLC system (GE Healthcare). The column was washed with 10 CV of the equilibration buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... each layered on 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess TCEP was removed using a NAP-5 column (GE Healthcare) pre-equilibrated with cold-degassed PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Excess dye was removed using NAP-5 columns (GE Life Sciences). Fractions containing ssDNA were identified using absorbance measurements at 260 nm ...
-
bioRxiv - Molecular Biology 2024Quote: ... and applied to 3 × 5-mL StrepTrap HP columns (GE Healthcare), connected in series ...
-
bioRxiv - Biochemistry 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
-
bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
-
bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
-
bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
-
bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
-
bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
-
bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
-
bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
-
bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
-
bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
-
bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
-
bioRxiv - Neuroscience 2024Quote: The binding kinetics of 5G2 and 1G11 to tau peptides encompassing amino acids 407-421 and 407-423 were undertaken through Biacore 2000 (GE Healthcare) using surface plasmon resonance analysis according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: WT ubiquitin was expressed in a tagless form and purified with the glacial acetic acid method followed by gravity cation exchange chromatography (GE Healthcare) and SEC as described previously.77
-
bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...
-
bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
-
bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
-
bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
-
bioRxiv - Systems Biology 2023Quote: ... for 2 min and detected on an ImageQuant (GE Healthcare). The proteins were quantified using ImageQuant TL software (GE Healthcare ...
-
bioRxiv - Neuroscience 2023Quote: ... Radiographic contrast agent (Omnipaque, 2% diluted in saline, GE Healthcare) was used in conjunction with an Elite CFD radiographic C-arm (GE Healthcare) ...
-
bioRxiv - Genomics 2022Quote: ... HRP-linked F(ab’)2 fragment (NA9340) from GE Healthcare/Amersham.
-
bioRxiv - Microbiology 2023Quote: ... Bacteria were imaged using API Deltavision 2 (Applied Precision Instruments) widefield microscope using YFP filter set at 100x magnification.
-
bioRxiv - Microbiology 2023Quote: ... HRP-linked F (ab’)2 Fragment (GE Healthcare Bio-Sciences) and 1-Step™ TMB ELISA Substrate Solutions (Thermo Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 µl 0.5 mM NiCl2 ...
-
bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
-
bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
-
bioRxiv - Biochemistry 2020Quote: ... Supernatant was applied to a 5 ml StrepTactin column (GE Healthcare, Sweden) and eluted with 2.5 mM desthiobiotin in 25 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: ... A total of 5 mL Ni2+ Sepharose 6 Fast Flow (GE Healthcare) was transferred to a 20 mL gravity flow column ...
-
bioRxiv - Biophysics 2020Quote: ... The mixture was loaded onto a MonoQ 5/50 column (GE Healthcare), and after washing the column with 100mM NaCl in 20 mM TRIS pH 7.0 mSAv-3xHis6 was eluted at 20 mM TRIS pH 7.0 ...
-
bioRxiv - Immunology 2020Quote: ... Buffer exchange was then performed using a NAP-5 column (GE Healthcare) and protein amount quantified using a MicroBeta TriLux liquid scintillation counter ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 mM DTT) and loaded onto a Mono S column (GE Healthcare). Bound proteins were eluted by linear NaCl gradient with buffer CL and buffer CH (20 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: ... filtered and loaded onto a HisTrap HP 5 ml column (GE Healthcare). After extensive washes with wash buffer (50 mM Hepes pH 8 ...
-
bioRxiv - Biochemistry 2020Quote: ... and manually loaded onto a 5 mL HiTrap Q column (GE Healthcare). The flow-through containing CDK12KD/CCNK∆C was concentration by ultrafiltration ...