Labshake search
Citations for GE Life Sciences :
351 - 400 of 2811 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
-
bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
-
bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
-
bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
-
bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
-
bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
-
bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
-
bioRxiv - Biochemistry 2023Quote: The DNA sequences encoding the DNA binding domains of Pdr1p (residues 1-207) and Pdr3p (residues 1-184) were amplified by PCR from the Yeast Genomic Tiling Collection (GE Healthcare YSC4613). The PCR products were inserted into the pMCSG7 vector by ligation independent cloning ...
-
bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
-
bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Immunology 2023Quote: ... to a ratio of 1 to 1 before being layered onto Ficoll Paque (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 x g for 25 min) ...
-
bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
-
bioRxiv - Neuroscience 2022Quote: ... distilled water was replaced with a 1:1 dilute of non-ionic iodine Omnipaque 350 contrast agent (Iohexol, 350 mg iodine/ml; GE Healthcare AS) in normal saline via 3 ml syringe ...
-
bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution; CtNo_NA931, GE Healthcare, Tokyo, Japan), developed with Immobilon Western Chemiluminescent HRP Substrate (CtNo_WBKLS0100 ...
-
bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed in TBST and incubated for 1 hour at room temperature with HRP-conjugated secondary antibody (1:10000 in PBS; GE Healthcare/Biocat) Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2024Quote: ... in PBS for 20 minutes then incubated in an excess of blocking solution (1% bovine serum albumin, Sigma-Aldrich, A7906; 1% inactivated horse serum, GE Healthcare B15-023 ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were reverse-transfected using DharmaFECT 1 (GE Healthcare) and then transfected again 24 h later according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... and sheep anti-mouse (NA931, GE Healthcare, 1:10000). The guinea-pig anti-KCTD16 antibody was raised against a synthetic peptide derived from mouse KCTD16 (aa7-23 ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 mL Q Sepharose® column (GE Healthcare 17051005) was charged with 1 M KCl and then equilibrated with elution buffer containing 250 mM imidazole ...
-
bioRxiv - Genomics 2020Quote: ... HRP-conjugated anti-mouse (GE Healthcare NA931V, 1:10000) and HRP-conjugated anti-rabbit (Santa Cruz sc-2004 1:5000 ...
-
bioRxiv - Biochemistry 2020Quote: ... a modified version of pGEX-4T-1 (GE Healthcare) where the thrombin site is replaced with a TEV cleavage site.
-
bioRxiv - Genomics 2021Quote: ... with 1 mL Histrap column (GE Healthcare Life Sciences) pre-equilibrated with buffer A ...
-
bioRxiv - Plant Biology 2021Quote: ... 1% (w/v) dithiothreitol (DTT; GE Healthcare, Piscataway, USA), and 10 μL of EDTA-free Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (GE Healthcare Cat# NA931, 1:5000 dilution), and then detected using an ECL SuperSignal West Femto y West Pico detection kit (Pierce).
-
bioRxiv - Cell Biology 2022Quote: ... Dharmacon DharmaFECT 1 transfection reagent (GE Healthcare, Manchester, UK) was used following the manufacturer’s siRNA transfection protocol with slight modification ...
-
bioRxiv - Developmental Biology 2022Quote: ... mouse IgG HRP linked (1:7,500, NXA931V, GE Healthcare). We used ECL Western Blotting detection reagent (RPN2232 and RPN2209 ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mM DTT using Akta FPLC system (GE Healthcare) at 0.2 mL/min ...
-
bioRxiv - Immunology 2021Quote: ... followed by anion exchange (1 ml HiTrapQ, GE Healthcare), according to manufacturers instructions ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) using a HiTrap column (GE Healthcare). The resulting eluate was passed over 1 mL Streptactin XT high capacity beads (IBA ...
-
bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... The pGEX-6P-1 vector (GE Healthcare, Uppsala, Sweden) was also amplified and modified by inverse PCR using primer pair For_PreSci_GST and Rev_PG_Met ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-rabbit conjugated HRP (1:500; GE Healthcare) as secondary antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... Vector Map: pLKO.1) were purchased from GE Healthcare Dharmacon ...
-
bioRxiv - Biochemistry 2021Quote: ... and a 1 ml HiTrap Q column (GE Healthcare), both equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.
-
bioRxiv - Physiology 2021Quote: ... followed by biotinylated antibody (GE Healthcare, RPN1004V, 1:200), HRP-labeled streptavidin (Southern Biotech ...
-
bioRxiv - Biophysics 2020Quote: ... through a 1 mL HisTrap HP column (GE Healthcare) and eluting with 350 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... and sheep anti-mouse IgG (GE Healthcare, 1:2000) HRP-conjugated secondary antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit IgG HRP (1:5000 GE Healthcare NA934V), anti-mouse IgG HRP (1:5000 Abcam ab97046).
-
bioRxiv - Biochemistry 2023Quote: ... or donkey anti-rabbit (1:10,000; GE Healthcare #NA934V).