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Citations for GE Life Sciences :
3901 - 3950 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Analytical size exclusion chromatography was performed with 20μl protein samples loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) mounted on an Agilent 1260 HPLC ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were concentrated ,applied to gel-filtration using a Superdex 75 prep grade HiLoad 16/60 column (GE Healthcare) equilibrated in buffer K (10 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Microbiology 2020Quote: ... The protein content during the washing and elution steps was monitored using the Äkta purifier 100 system (GE Healthcare, UK) and double-checked with a Bradford assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... Pure proteins with the correct homooligomeric conformation were collected from a Superose 6 10/300 GL SEC column (GE Healthcare) in Tris-buffer (TBS ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-containing fractions were then concentrated by microfiltration before loading on a Superdex 200 26/60 prepgrade column (GE Healthcare) column that had been equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 Column (GE Life Sciences).
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected and the antibodies were purified on a HiTrap Protein-A column (GE healthcare, Little Chalfont, UK).
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were pooled and 1.6 ml protein was subjected to size exclusion chromatography using a Superdex 200 XK16/60 column (GE Healthcare) and equilibrated into 50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
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bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein extracts underwent vacuum filtration through a pre-wet 0.2 μm pore size nitrocellulose membrane (GE Healthcare) for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Concentrated and filtered protein was analyzed by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with HEPES buffer ...
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bioRxiv - Molecular Biology 2021Quote: Proteins (10 μg) were separated by 10%–12.5% SDS-PAGE and electroblotted onto a 45-μm nitrocellulose membrane (GE Healthcare). Membranes were stained with Ponceau to ensure equal distribution of proteins in each lane ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgM were separated from serum by affinity chromatography using HiTrap Protein G High Performance column (Cytiva, GE Healthcare) and CaptureSelect IgM Affinity Matrix (Thermofisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting protein samples were further purified by size-exclusion chromatography using a Superdex 75 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Bioengineering 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography on a Hiload 16/600 Superdex 75 pg column (GE Healthcare) in PBS buffer.
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bioRxiv - Biochemistry 2021Quote: ... The unbound protein was concentrated and further purified via size exclusion chromatography on HiLoad S200 16/60 column (GE Healthcare) in buffer A2 (300 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Cancer Biology 2022Quote: MSLN complex with VH 3C9 was prepared by injecting the protein mixture to Superdex 75 column (GE Healthcare, Chicago IL), and concentrated to 12 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was applied onto superdex200 increase 10/300 GL column via fast protein liquid chromatography (AKTA Pure, GE Healthcare) with buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μl of pre-equilibrated protein L beads (Capto™ L resin, GE Healthcare Cat no. 17547802 Cat. no. 17547803) was added and reaction was incubated for 90 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were concentrated and further purified by size exclusion chromatography on a Superdex 75 column (GE Healthcare Life Sciences) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the crude lysate proteins were separated by Tricine-SDS PAGE (16%) and blotted onto a PVDF membrane (AmerhamTMHybondTM, 0.2 µM PVDF; GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...