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Citations for GE Life Sciences :
3901 - 3950 of 4126 citations for Japanese Encephalitis Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions with low protein background were pooled and subjected to size-exclusion chromatography on a Superdex 75 pg column (GE Healthcare, USA) in the storage buffer (DesAOMT ...
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bioRxiv - Microbiology 2023Quote: ... The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant; #28-9670-62, GE Healthcare) using binding buffer pH 7 ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Genetics 2023Quote: ... Peaks containing the ThsA protein were injected to a size-exclusion chromatography (SEC) column (Superdex 200_16/60, GE Healthcare, 28-9893-35) equilibrated with SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8x-His-SUMO tag was removed by Ni-NTA agarose beads and the proteins were further purified by size exclusion chromatography using Superdex 75 16/600 (GE Life Sciences) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using, ECL prime (Amersham, Uppsala, Sweden) and detected using ImageQuant™ LAS 4000(GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant LMW-SLP proteins from the soluble fraction were purified by affinity chromatography on Ni-agarose columns using an AKTA prime (GE Healthcare, #11001313). Proteins were dialyzed against 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Molecular Biology 2023Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: The His6-tagged proteins were purified by affinity chromatography using HisTrap™ HP Ni-NTA columns and the ÄKTA purifier liquid chromatography system (GE Healthcare) according to the instructions of the manufacturers ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Cell Biology 2024Quote: ... the protein signal was developed by Super Signal West Femto (Thermo 34095) and visualized with Amercham Hyperfilm ECL system (GE Healthcare 28906839) film.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS– PAGE) and proteins were transferred into nitrocellulose membranes (Amersham Protran Premium 0.45μm nitrocellulose blotting membranes, GE Healthcare Life Sciences, USA). For immunostaining ...
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bioRxiv - Biochemistry 2024Quote: ... The purification of Ubi-SSFc and His6-SSFc was performed using HisTrap resin and AKTA protein purification system (Ge Healthcare Life Sciences). Firstly ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Immunology 2021Quote: ... After washing the membranes with TBST, protein bands were detected with a chemiluminescent substrate (BIOMAX, USA) through a CCD Imager (GE life sciences, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified from the infected cell culture supernatants by binding to a HiTrap ProteinG HP column (GE Healthcare Life Sciences, Piscataway, NJ) and eluted with 0.1 M citrate ...
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bioRxiv - Biophysics 2022Quote: ... After purification with Ni-NTA agarose the protein was diluted to 375mM NaCl and further purified on an ÄKTA purifier system (GE Healthcare Life Sciences) at 4 °C using 1ml HiTrap™ Heparin HP column (Cytiva) ...
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bioRxiv - Microbiology 2019Quote: ... YpdAC14A and Tpx-roGFP2 proteins were purified using His Trap™ HP Ni-NTA columns (5 ml; GE Healthcare, Chalfont St Giles, UK) and the ÄKTA purifier liquid chromatography system (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Biophysics 2019Quote: ... for 20 min at 4 0C and histidine tagged protein was recovered from the lysate using a HiTrap TALON Crude Co2+ column (GE Healthcare, Piscataway, NJ) as described by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2019Quote: ... Lysates (10-40 μg/lane) were separated by SDS-PAGE and protein was transferred onto a polyvinylidene fluoride (PVDF) membrane (Hybond-P, GE Healthcare, Uppsala, Sweden). Specific primary antibodies (see Antibodies subsection ...
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified according to their size on a HiLoad™ 16/60 Superdex™ 200 prep grade prepacked column (GE Healthcare) using an ÄKTAprime chromatography system at 4°C with a SEC buffer containing 50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We pooled fractions containing the purified protein, desalted the pooled fractions using PD-10 desalting columns packed with Sephadex G-25 resin (17085101, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
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bioRxiv - Biophysics 2020Quote: ... SHP-1 was further purified by size-exclusion chromatography on an AKTA fast protein liquid chromatography system equipped with a Superdex S200 10/300 GL column (both from GE Healthcare Life Sciences) equilibrated with 10 mM Hepes (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: Extracted proteins of three-day-old sexual fungal tissues were separated by PAGE and transferred onto a nitrocellulose membrane (GE Healthcare, Wisconsin, USA) as described (Schinke et al. ...
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bioRxiv - Neuroscience 2020Quote: ... and 25μg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Neuroscience 2020Quote: ... The remaining elution buffer from the protein purification process was substituted by PBS using sephadex-based PD MidiTrap G-25 columns (GE Healthcare, Chicago, USA). Afterwards ...
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bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and samples were pre-cleared for 1 hour using protein G sepharose (GE Healthcare, 17-0618-01). Immunoprecipitation was carried out overnight at 4 °C using anti-HA agarose beads (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants from both approaches were filtered and mAbs were purified from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...