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Citations for GE Life Sciences :
3751 - 3800 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Fluorescence profiles and peak volumes of labeled proteins were obtained using ImageQuant TL v2005 (GE Healthcare Life Sciences, Piscataway, NJ). For calculation of AA74-1 IC50 values (Fig ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was purified by size exclusion chromatography over a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the protein of interest was enriched via metal ion affinity chromatography (Ni-NTA) resin (IMAC, 5ml HiTrap Chelating, GE Healthcare). After 10 column washes with (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: A volume of 25-30 µL serum was spotted onto a Whatman® Protein Saver™ 903 Card (GE Healthcare), air-dried at room temperature for approximately 2 hours and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... Processed protein was concentrated and further purified on a HiLoad Superdex 200 16/60 size exclusion chromatography column (GE Healthcare) equilibrated in 20 mM Tris HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Refolded protein was concentrated (Vivaspin or Amicon 10 kDa) and further purified on Superdex S75 or S200 columns (GE Healthcare) in SEC buffer as indicated in Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were clarified by centrifugation at 12,000 × g for 15 min and GST-Rab proteins were applied at saturating levels to glutathione Sepharose beads (GE Healthcare) for 30 min at 4°C and then washed extensively to remove unbound material ...
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bioRxiv - Immunology 2021Quote: ... TCR and pMHC proteins were dialyzed and further purified via anion exchange chromatography (HiTrap Q HP; Mono Q; GE Healthcare) and size-exclusion (Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was separated from free D3_Sof protein by size-exclusion chromatography using “Superdex 75 10/300 GL” column (GE Healthcare) at 0.4 ml/min flow rate using running buffer containing 25 mM Tris-HCl (pH 7.6 at 25 ° C) ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... and protein further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (GE Life Sciences), 1.0 mL/min in Na2HPO4 pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the bound fusion protein was eluted with 20– 200 mM imidazole gradient using a FPLC purifier system (ÄKTA; GE Healthcare). Peak fractions were pooled and digested with SENP2 protease (at final 0.01 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was further purified over a gel filtration column (HiPrep 16/60 Sephacryl S-100 High Resolution, GE Healthcare) equilibrated with AUC buffer (50 mM NaH2PO4 pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 100-400 mM NaCl (depending on the protein) with either PD-10 or HiPrep 26/10 desalting columns (GE Healthcare). SGTA ...
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bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
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bioRxiv - Biophysics 2020Quote: ... Supernant containing soluble proteins was filtered using a 0.45 μm device and applied to a HisTrap HP affinity column (GE Healthcare) equilibrated in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The complex protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cleared lysates containing the protein of interest were incubated 4h at 4°C with 1.5 μM of the indicated GST-fused proteins and 30 μl of glutathione-sepharose CL-4B beads (GE Healthcare). After three washes in lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Biochemistry 2020Quote: ... purified proteins were mixed in a stoichiometric ratio and loaded on a SD200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were eluted from a Superdex 200 Increase 10/300 GL column using an ÄKTA Pure FPLC (GE Healthcare) at a flow rate of 0.7 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were applied to a size-exclusion column (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) with the buffer containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was washed again and proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Final purification of GST-CsrA protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were further purified via size exclusion chromatography using a Superdex™ 200 Increase 10/300 GL (GE Healthcare) with 50 mM Tris/HCl buffer containing 100 mM KCl and 0.5 mM EDTA.
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bioRxiv - Microbiology 2020Quote: ... Eluted sample was further purified with fast protein liquid chromatography (FPLC) and gel filtration on a Superdex200 column (GE Healthcare) in sizing buffer (30 mM Tris·HCl ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by SEC (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A with pH 7.5 as eluent ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was expressed using ExpiCHO expression system (LifeTechnologies) according to the manufacturer’s protocol and purified using a Protein A MabSelect SuRE column (GE Healthcare). The wash buffer contained 20mM Tris & 150mM NaCl buffered to pH 8.6 and the elution was done using 0.1 M Citric acid pH 2.5 ...
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...