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Citations for GE Life Sciences :
3701 - 3750 of 4868 citations for Glutathione Colorimetric Detection Kit 200 High Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 1.5 μM PCNA trimer and 20 μM TTP was loaded onto a Superdex 200 increase 3.2/300 column (GE Life Sciences) equilibrated with a buffer comprising [25 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Images were acquired with 200 ms exposure time every 4 minutes for at least 6 h at 37°C using Softworx (Applied Precision) software ...
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Biophysics 2019Quote: ... A Wyatt QELS dynamic light scattering module for determination of hydrodynamic radius and an Optilab T-rEX refractometer (Wyatt Technology) were used in-line with a size exclusion chromatography analytical column (Superdex 200 HR10/300, GE Healthcare) [35] ...
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bioRxiv - Microbiology 2019Quote: ... BepA protein was concentrated to ~60 mg/ml by ultra-filtration and then further purified on a HiLoad Superdex 200 26/600 column (GE Healthcare) equilibrated in buffer D ...
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bioRxiv - Microbiology 2019Quote: ... Samples were further purified by gel filtration chromatography using either a Superdex 75 (26/60) or Superdex 200 (26/60) column attached to an AKTA-format FPLC (GE Healthcare) and PBS as a running buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was loaded at concentrations ranging from 2 to 16 mg/ml on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... All affinity-purified proteins were size-exclusion-purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare, catalog 28990944) in HEPES buffered saline (50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... A 50-μl protein sample (1-2 mg/ml) was analyzed on a Superdex S-200 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were further purified by size-exclusion chromatography (on either a Superdex 200 increase 10/300 GL or a HiLoad 16/600 Superdex 200pg column, both GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The HiTrap ButylFF column of 1 mL and the gel filtration column Superdex 200 10/300 GL were from GE Healthcare. MacroPrep HighS resin was from Biorad ...
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bioRxiv - Biochemistry 2019Quote: ... His6-MBP-TEV-NRBF2MIT was concentrated with a 10 kDa MWCO Amicon concentrator and injected over a Superdex-200 column (GE Healthcare) equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The final purification step for all variants encompassed a size exclusion chromatography run (HiLoad 16/60 or 26/60 Superdex 75 or 200 prepgrade, GE Healthcare) using a buffer with 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... The eluted BBSome:ARL6 complex was concentrated to 500 μl using a concentrator with a 100 kDa molecular weight cutoff (Thermo Fischer Scientific) and injected onto a Superdex 200 (16/600) size-exclusion chromatography column (GE Healthcare) equilibrated with 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and cleaved protein was concentrated and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the eluted protein were concentrated with a 30 kDa molecular mass cutoff centrifuge concentrator (Amicon) and loaded onto a HiLoad 26/60 Superdex-200 size-exclusion column (GE Healthcare) pre-equilibrated with 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and Waters 410 differential refractometer (Waters) in line with an ÄKTA pure chromatography system fitted with a Superdex 200 Increase column (both GE Healthcare). Data was analysed using ASTRA 5.3.4 software (Wyatt Technology).
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bioRxiv - Physiology 2019Quote: ... which was purified via sequential protein-A affinity and size exclusion chromatography (SEC) techniques using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare), as described elsewhere (Suriben et al ...
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bioRxiv - Physiology 2019Quote: ... and purified from conditioned media by sequential protein-A affinity and size-exclusion chromatographic (SEC) methods using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare). All purified antibody material was verified endotoxin-free and formulated in PBS for in vitro and in vivo studies ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... Fractions passed through the HisTrap HP column were collected for further purification with the Superdex 200 Increase 10/300 GL column (GE Healthcare).
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The reaction mixture was then used for purification through size-exclusion chromatography with Superdex 200 Increase 10/300 GL column (GE Healthcare) in buffer A to remove extra σ28 protein and nucleic acids.
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bioRxiv - Biochemistry 2020Quote: ... the V2R was purified by size exclusion chromatography (SEC) using a Superdex 200 (10/300 column) connected to an AKTA purifier system (GE Healthcare). Fractions corresponding to the pure monomeric receptor were pooled (~ 2 ml ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was concentrated in a 100 kDa cut-off Vivaspin® 2 Centrifugal Concentrator to a final volume of less than 500 μl before injected onto a Superdex 200 10/300 Increase GL column (GE Healthcare), which was equilibrated with GF buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... with a molecular weight cut-off (MWCO) of 30 kDa and polished by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Biophysics 2021Quote: ... during His6-tag cleavage to remove phosphate groups resulting in inhibitory autophosphorylation ([19]. Size exclusion chromatography was carried out using a Superdex 200 16/600 column (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... and 20 mM NaCl and subjected to gel filtration using the same buffer on a Superdex 200 column attached to an ÄKTA FPLC (GE Healthcare) at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were further purified by size exclusion chromatography (SEC) using the HiLoad Superdex 16/600 200 prep grade column (GE Healthcare) run in 20 mM Tris-HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The expressed GP was purified using an mAB100 antibody column followed by SEC on a HiLoad Superdex 200 16/600 column (GE Healthcare). PBS (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated to 2 mL and further purified by size-exclusion chromatography using HiLoad 16/600 Superdex 200 (Ge healthcare).
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 concentrators and further separated by HiTrap Heparin HP column and Hi-load 16/600 superdex 200 column (GE Healthcare). Fractions from the column were pooled and subjected for SEC-MALS analysis to determine their oligomeric status ...
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bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... BSA was used for calibration and proteins were separated on a Superdex 200 Increase 10/300 analytical SEC column (GE Healthcare) with a flow rate of 0.4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Biochemistry 2021Quote: ... Nb was mixed with the target protein in 1.2-1.5 fold molar excess and the complex isolated via SEC on a Superdex 75 Increase 10/300 GL or Superdex 200 Increase 10/300 GL (GE healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and Sharpin were eluted and further purified over a Superdex 75 16/600 pd or a Superdex 200 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted from Histrap HP column was further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... Both the His-tagged and GST-tagged variants of PrgA were subsequently loaded on a Superdex- 200 16/600 size exclusion chromatography column (GE Healthcare) in 10 mM HEPES/NaOH (pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... ExoU was purified by an initial affinity step (immobilized nickel affinity chromatography) followed by size-exclusion chromatography (16/600 Superdex 200 GE healthcare) in 20 mM Tris-HCl pH 8.2 ...
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bioRxiv - Biochemistry 2020Quote: ... then followed by size exclusion with either a Superdex 75 or Superdex 200 column in an AKTA pure FPLC (GE Healthcare). The final protein product was stored in buffer containing 20 mM Tris pH 7.3 ...
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bioRxiv - Bioengineering 2020Quote: ... concentrated with 100 kDa Amicon centrifugal filter units and injected into a size exclusion HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated with storage buffer G (20 mM HEPES-KOH pH 7.5 ...