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Citations for GE Life Sciences :
3701 - 3750 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The protein was eluted with IMAC buffer containing 330 mM imidazole and loaded onto a HiTrap SP HP column (GE Healthcare). The column was washed with ion exchange buffer (IEC ...
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bioRxiv - Microbiology 2019Quote: Samples were electrophoresed on a 15% SDS polyacrylamide gel and proteins were then blotted onto a nitrocellulose membrane (GE Healthcare, Amersham). Nitrocellulose was blocked with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Physiology 2019Quote: ... which was purified via sequential protein-A affinity and size exclusion chromatography (SEC) techniques using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare), as described elsewhere (Suriben et al ...
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bioRxiv - Physiology 2019Quote: ... and purified from conditioned media by sequential protein-A affinity and size-exclusion chromatographic (SEC) methods using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare). All purified antibody material was verified endotoxin-free and formulated in PBS for in vitro and in vivo studies ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through that contained the untagged protein of interest was applied to an ion exchange chromatography HiTrap Q column (GE Healthcare). The unbound protein sample ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Genetics 2020Quote: ... The cap-enriched mRNA was prepared from 100 μg of total RNA with GST-eIF4EK119A protein and glutathione sepharose 4B (GE Healthcare), as described previously (Bilska et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were further purified by size exclusion chromatography (SEC) using the HiLoad Superdex 16/600 200 prep grade column (GE Healthcare) run in 20 mM Tris-HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated to 2 mL and further purified by size-exclusion chromatography using HiLoad 16/600 Superdex 200 (Ge healthcare).
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bioRxiv - Biochemistry 2020Quote: ... BSA was used for calibration and proteins were separated on a Superdex 200 Increase 10/300 analytical SEC column (GE Healthcare) with a flow rate of 0.4 ml/min ...
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bioRxiv - Biochemistry 2020Quote: Elution peaks corresponding to a molar mass of a dodecameric or dimeric protein of PrgL32-208 were further analysed by SEC-MALS with the use of an ÄKTApure system (GE Healthcare) coupled to a miniDAWN TREOS II detector and an OptiLab T-rEX online refractive index detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... or 100 to 300 µg samples of individual proteins were injected onto a Superdex 75 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM TrisHCl ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with 10 mM maltose and further purified via gel filtration on a Superdex S75 (GE Healthcare) column.
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μg of each fusion protein were radioiodinated with 125I and Chloramine T hydrate and purified on a PD10 column (GE Healthcare), as described previously [38] ...
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Bioengineering 2020Quote: ... Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% (w/v ...
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bioRxiv - Bioengineering 2020Quote: ... Unconjugated molecules were separated using size exclusion chromatohraphy with Superdex 200 Increase 10/300 GL column on ÄKTA fast protein liquid chromatography (FPLC, GE Healthcare). The purified nanosensors were concentrated by spin filters (MWCO = 10 kDa ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by loading the supernatant onto a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) equilibrated with 5 CV of 95% Buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The gels were exposed to imaging plates and the images of separated tracer-labeled proteins were obtained by reading the plate using a Typhoon 9410 (GE Healthcare) image analyzer ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling of SNAP fusion proteins and depletion of free dye was confirmed by SDS-PAGE followed by fluorescence imaging on a Typhoon imager (GE Healthcare). Extracts were checked to verify transcription activity (e.g. ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was diluted with lysis buffer before application to a 5ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer and eluted using buffer supplemented with 2 mM Desthiobiotin.
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bioRxiv - Biochemistry 2020Quote: 100-500 µl samples (lipid-bound and lipid-free ApoE3 and ApoE380-255) were applied to an AKTA protein purification system (GE) equipped with a Superdex 200 10/300 (GE healthcare) column and a 500 µl sample loop and run at 0.5 ml/min at 5°C ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... were separated onto a SDS-PAGE and proteins were transferred to Hybond-P PVDF transfer membrane (GE Healthcare, Amersham™ RPN303F) using the manufacturer’s instructions and a Trans-Blot TURBO Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The desalted elution fraction was further purified using fast protein liquid chromatography (FPLC) with a HiLoad 16/60 Superdex 200 size exclusion column (GE Healthcare). The buffer used for laforin purification for the small-scale pulldowns described in methods 2.3.2 was (50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were affinity purified on an amylose column and were further purified by ion-exchange chromatography (HiTrap Q HP, GE Healthcare). Protein concentrations were determined by UV spectroscopy ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated pNephrin was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated Cas was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Biotech AB/Sweden) prior to protein separation on a S200 Superdex 10/300GL column on an Äkta Purifier (both GE Healthcare). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant for purified FliWWT/N108Y/K123E/Q124R protein was further cleaned by size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...