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Citations for GE Life Sciences :
3601 - 3650 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Protein samples were concentrated and further purified by size exclusion chromatography on a Superdex 75 column (GE Healthcare Life Sciences) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the crude lysate proteins were separated by Tricine-SDS PAGE (16%) and blotted onto a PVDF membrane (AmerhamTMHybondTM, 0.2 µM PVDF; GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was loaded into a HiTrapTm Q HP anion exchange column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and eluted with ∼ 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with buffer E and concentrated to 10 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 4°C at 10,000 g for 30 minutes and the supernatants were used to purify target proteins by Ni NTA agarose (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were further purified via size exclusion chromatography using a GE Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... then separated by fast protein liquid chromatography system using HiTrap SP HP column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and proteins were transferred to Cytiva Amersham™ Protran™ 0.45 µm NC nitrocellulose blotting membranes (GE Healthcare, Munich, Germany). After blocking with 5% bovine serum albumin (Roth ...
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bioRxiv - Immunology 2024Quote: ... The complexes of SH-01AF594 with C3d and Fab 3E12 were obtained by concentrating ∼1 mg of either protein with 300µg of SH-01AF594 to ∼200µL before removing the excess protein via SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS ...
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bioRxiv - Biochemistry 2024Quote: ... with the exception that the protein eluted from the Ni-NTA column was loaded on HiTrap Heparin column (GE Healthcare) equilibrated with HP1 buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated and loaded onto a S75 16/600 size exclusion chromatography column (GE Healthcare, Chicago, USA). After chromatography ...
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bioRxiv - Molecular Biology 2024Quote: For CD spectroscopy and SAXS measurement (see below) proteins were additionally purified using gel filtration chromatography (ÄKTA purifier, GE Healthcare) on a semi-preparative Superdex™ 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was concentrated with a 30kDa cutoff filter (Amicon) and loaded onto a S200 10/300 column (GE Healthcare) equilibrated in SEC buffer (50mM Tris-HCl pH 7.5 @RT ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified using size exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... Bocillin-labelled protein samples were run through 15% SDS-PAGE and visualized under a Typhoon FLA 7000 (GE Healthcare, USA) at an excitation wavelength of 488 nm and an emission wavelength of 526 nm ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... and proteins were collected from culture supernatants and purified using the HisTrap HP column (GE Healthcare Life Sciences, Pittsburgh, PA) as described previously 33 ...
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bioRxiv - Microbiology 2024Quote: ... the labeled proteins were verified by running an SDS-PAGE and scanning using a Typhoon scanner (GE Healthcare, Danderyd, Sweden).
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... The fraction bound was measured by capturing protein-RNA complexes on Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) and unbound RNA on a Nylon XL membrane (GE Healthcare Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... and the protein from the other gel was transferred to a 0.45-mm-pore-size nitrocellulose membrane (Hybond-ECL; GE Healthcare) and incubated with mouse α-HIS antibody (Genscript A00186) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts (30 µg) were separated by SDS-PAGE and transferred to Hybond-C Extra nitrocellulose membranes (GE Healthcare, USA). Details regarding primary and secondary antibodies are listed in Table S5 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were concentrated and subjected to size exclusion chromatography (Superdex™ 200 Increase 10/300, GE Healthcare, ÄKTA pure 25M) in 25 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... detection of the protein bands was achieved using either the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare, #RPN2232) or the Amersham™ ECL Select™ Western Blotting Detection Reagent (#RPN2235) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated according to size using standard SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare, Amersham, Germany). Membranes were blocked in 5 % skimmed milk/TBS-T and probed with primary antibodies (listed in supplementary table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cat. No. 10600001; GE Healthcare; Chicago, IL, 16 USA). The membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted proteins were further purified over gel filtration at 4°C on a Superdex 200 10/300 GL (GE Healthcare) column the using lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...