Labshake search
Citations for GE Life Sciences :
3601 - 3650 of 4565 citations for Recombinant Human CD19 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... 100-400 mM NaCl (depending on the protein) with either PD-10 or HiPrep 26/10 desalting columns (GE Healthcare). SGTA ...
-
bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
-
bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
-
bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
-
bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
-
bioRxiv - Biophysics 2020Quote: ... Supernant containing soluble proteins was filtered using a 0.45 μm device and applied to a HisTrap HP affinity column (GE Healthcare) equilibrated in buffer A (40 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biophysics 2021Quote: ... The complex protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
-
bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cleared lysates containing the protein of interest were incubated 4h at 4°C with 1.5 μM of the indicated GST-fused proteins and 30 μl of glutathione-sepharose CL-4B beads (GE Healthcare). After three washes in lysis buffer ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences).
-
bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... purified proteins were mixed in a stoichiometric ratio and loaded on a SD200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: Protein samples were eluted from a Superdex 200 Increase 10/300 GL column using an ÄKTA Pure FPLC (GE Healthcare) at a flow rate of 0.7 mL/min ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were applied to a size-exclusion column (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) with the buffer containing 50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
-
bioRxiv - Plant Biology 2020Quote: ... The blot was washed again and proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... Final purification of GST-CsrA protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... the proteins were further purified via size exclusion chromatography using a Superdex™ 200 Increase 10/300 GL (GE Healthcare) with 50 mM Tris/HCl buffer containing 100 mM KCl and 0.5 mM EDTA.
-
bioRxiv - Microbiology 2020Quote: ... Eluted sample was further purified with fast protein liquid chromatography (FPLC) and gel filtration on a Superdex200 column (GE Healthcare) in sizing buffer (30 mM Tris·HCl ...
-
bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
-
bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by SEC (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A with pH 7.5 as eluent ...
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
-
bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
-
bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
-
bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
-
bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody was expressed using ExpiCHO expression system (LifeTechnologies) according to the manufacturer’s protocol and purified using a Protein A MabSelect SuRE column (GE Healthcare). The wash buffer contained 20mM Tris & 150mM NaCl buffered to pH 8.6 and the elution was done using 0.1 M Citric acid pH 2.5 ...
-
bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by transfer of proteins from gel to polyvinylidene fluoride (PVDF) membrane (Amersham Hybond P 0.45, GE Healthcare Life Sciences) and sequential use of primary and secondary antibodies that are listed in Table 3 ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
-
bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by sizeexclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer B with 0.06% (w/v ...
-
bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
-
bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex200 16/60 HiLoad gel filtration column (GE Healthcare). For CLGltPh ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against phospho-Ser935 LRRK2 rabbit monoclonal (1/1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 10% Tris-Tricine gels and imaged using a Typhoon 9410 Molecular Imager (GE Healthcare Life Sciences).
-
bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
-
bioRxiv - Neuroscience 2022Quote: ... We performed size exclusion chromatography on the eluted proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
-
bioRxiv - Neuroscience 2022Quote: ... we performed size exclusion chromatography on the concentrated proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
-
bioRxiv - Microbiology 2022Quote: ... The protein was further purified by size exclusion chromatography on HiLoad 26/600 Superdex 200 prep grade column (GE Healthcare) equilibrated with the working buffer (50 mM Tris-HCl pH 8.5 ...
-
bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... TEV protease was added during dialysis to remove the N-terminal His6-tag.The protein was retrieved from dialysis and loaded onto a 1ml HiTrap SP HP IEX column (GE Healthcare) and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0 ...
-
bioRxiv - Biophysics 2022Quote: ... Protein eluted from the affinity columns was further purified by size-exclusion chromatography using a Superdex 75 column (GE healthcare).
-
bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...