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Citations for GE Life Sciences :
3601 - 3650 of 4670 citations for Rat Androgen dependent TPF1 regulating protein ADTRP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Analytical size exclusion chromatography showed that the majority of the protein eluted in a peak at 13 mL from a Superdex 200 column (GE Healthcare). BacILT7 was dialysed against HBS-PE and cleared by centrifugation at 100,000 g for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... The components were ran electrophoretically and the proteins were electrotransferred to a polyvinylidene difluoride membrane (Hybond-P™, Amersham GE Healthcare) at 50 V and a current of 90 mA overnight using a transfer buffer (1.9% Tris base ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Zoology 2019Quote: ... The rehydrated IPG strips containing 100 μg of proteins were then subjected to isoelectric focusing as per the manufacturer’s standard protocol (GE Healthcare, USA). After IEF ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer E (20 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer B ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Microbiology 2019Quote: ... the His6-tag was removed using TEV protease and the protein was subjected to size exclusion chromatography (HiPrep 26/60 Sephacryl S-200; GE Healthcare) using a buffer consisting of 40 mM HEPES pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... For the in vitro ATPase activity assay and pull-down assay the buffer of the proteins was exchanged via a PD SpinTrap G-25 spin columns (GE Healthcare) to activity assay buffer (25mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting SNX9 and His6x-β2-hinge+appendage proteins were separately applied to a HiLoad 26/600 Superdex 200 pg column (GE Healthcare). Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a cation exchange HiTrap SP HP 5 ml column (GE healthcare life sciences) pre-equilibrated with 5 column volumes of buffer ASP (100 mM Ammonium acetate ...
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bioRxiv - Biochemistry 2019Quote: Nucleotide concentrations of an ongoing enzymatic reaction were determined in real-time by fast protein liquid chromatography (FPLC) using an ÄKTA Purifier instrument (GE Healthcare) equipped with a 1 mL Resource Q anion exchange (AEX ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation performed from whole cell extracts of NIH3T3-II10 and NIH3T3-K2 cells were following the same procedures with protein G Sepharose beads (GE Healthcare): 18 μg rabbit anti-TBPL2 (3024 ...
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bioRxiv - Microbiology 2020Quote: ... The protein in the supernatant was sequentially purified using a Ni-NTA column and a Superdex200 gel filtration column (GE Healthcare) as previously described (20) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were further purified by size exclusion chromatography (SEC) on a Superdex 75 Increase 10/300 GL column (GE Healthcare) for E2 cores and on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were further purified from aggregation products by size exclusion chromatography on a Superose 6 increase column (GE Healthcare) equilibrated with size exclusion buffer (10 mM HEPES pH 7.8 ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to remove remaining fluorophores and aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Protein eluates were then concentrated and buffer exchanged to PBS using Vivaspin filters with the appropriate molecular weight cutoff (GE Healthcare). Protein concentrations were determined by the Nanodrop method using the proteins peptidic molecular weight and extinction coefficient as determined by the online ExPASy software (ProtParam).
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bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Molecular Biology 2021Quote: ... total protein (50 μg) was subjected to SDS-PAGE followed by transfer to polyvinylidene difluoride membrane (GE Healthcare Life Sciences, Europe) using a semidry trans-blotter (BioRad ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing target protein were pooled and exchanged back to 100% buffer A using a Sephadex desalting column (HiPrep 26/10 column, GE Healthcare). TEV cleavage was performed overnight at 23°C with 1 mM DTT ...
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bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was monitored by Coomassie staining of SDS-PAGE and size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...