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Citations for GE Life Sciences :
3551 - 3600 of 4118 citations for Tyr0 pTH Related Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was monitored by Coomassie staining of SDS-PAGE and size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Immunology 2020Quote: ... The soluble xfR5 mAb from the supernatant was isolated using HiTrap™ Protein-A HP prepacked column (GE Healthcare; NJ, USA) following standard manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... Protein samples (each protein at a concentration of 40 μM) were loaded to a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with assay buffer (same with ITC).
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bioRxiv - Molecular Biology 2019Quote: ... Both the cleaved His-tag and the His-tagged protease were removed from protein solutions by passage over loose nickel beads (GE Healthcare). The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... Dialyzed protein was diluted two-fold in buffer Q1 and loaded onto a HiTrap Q HP anion exchange chromatography column (GE Healthcare), equilibrated in buffer Q1 ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membranes were washed three times with TBS-T and protein bands were visualized using the CDP-star detection reagent (GE Healthcare). Intensity was detected and quantitatively analyzed by the Fluor-S MAX multi-imager system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions of protein were then concentrated and loaded onto a 10/300 Superdex200 Increase gel filtration column (GE Life Sciences) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the TTPN protein was concentrated and loaded on a size-exclusion chromatography (SEC) column (HiLoad 26/60 Superdex 75 pg, GE Healthcare). Peak fractions from the first SEC step ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... fused to lysozyme and BRD4-ET either alone or together was evaluated by injecting each purified protein onto a Superdex 75 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with 20 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualised using ECL™ or ECL plus™ reagents and Hyperfilm® ECL (all from GE Healthcare) and detected with a CCD camera (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Immunology 2022Quote: SEC-MALS experiments were carried out using a Wyatt Dawn Heleos II multiangle light scattering detector (Wyatt Technology) coupled to an AKTA Purifier UPC10 FPLC protein purification system and a Superdex 200 size exclusion column (GE Healthcare). Protein molecular weights of the individual peaks observed in the size-exclusion chromatograms were analyzed by static light scattering (SLS ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was further purified using size-exclusion chromatography over a Superdex S75 13/300 or 16/600 column (GE Healthcare). For Ube2k-UBC containing the Avi tag ...
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bioRxiv - Cancer Biology 2021Quote: ... were used for the protein detection and membrane images were taken using Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL).
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bioRxiv - Microbiology 2021Quote: Oligomerization of delVirB4_CTn4 and delVirD4_CTn4 proteins was studied by gel chromatography on Superdex200 10/30 GL column connected to ÄKTA Explorer liquid chromatography system (GE Healthcare). The proteins ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was further purified by a HiLoad® 16/600 Superdex® 200 pg gel filtration column (GE Healthcare) in buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted protein was loaded onto a size exclusion chromatography column (Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) depending on protein size ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... All purification steps were carried out at 4°C using the ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE healthcare). Protein purity was assessed by SDS-PAGE ...