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Citations for GE Life Sciences :
3551 - 3600 of 4264 citations for Recombinant Human PTHLH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... were separated onto a SDS-PAGE and proteins were transferred to Hybond-P PVDF transfer membrane (GE Healthcare, Amersham™ RPN303F) using the manufacturer’s instructions and a Trans-Blot TURBO Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The desalted elution fraction was further purified using fast protein liquid chromatography (FPLC) with a HiLoad 16/60 Superdex 200 size exclusion column (GE Healthcare). The buffer used for laforin purification for the small-scale pulldowns described in methods 2.3.2 was (50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were affinity purified on an amylose column and were further purified by ion-exchange chromatography (HiTrap Q HP, GE Healthcare). Protein concentrations were determined by UV spectroscopy ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated pNephrin was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated Cas was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Biotech AB/Sweden) prior to protein separation on a S200 Superdex 10/300GL column on an Äkta Purifier (both GE Healthcare). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant for purified FliWWT/N108Y/K123E/Q124R protein was further cleaned by size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... His6-SUMO-FliWWT/N108Y/K123E/Q124R protein was further cleaned by size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein used in this study was overexpressed in pET28a and then purified by fast protein liquid chromatography (FPLC; AKTA Explorer 100, GE Healthcare)[19] ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... His- or GST-tagged proteins were captured from the lysate by the addition of 100 μL Ni Sepharose High Performance beads (GE Healthcare) or Glutathione Sepharose High Performance beads (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) in a 25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Diluted and filtered samples were then added to a Protein G High Performance MultiTrap 96-well plate (GE Healthcare, Chicago, IL) and shaken at 1,100 RPM for one hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2021Quote: ... A 12-20% gradient Tris-Glycine SDS-PAGE was used to separate labelled proteins and dried for exposure with a phosphorimager screen and scanned with a Typhoon 9400 or Typhoon FLA 7000 (GE Healthcare) for quantification ...
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bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2021Quote: Purified trypsinized post-fusion hMPV B2 F protein was subjected to size exclusion chromatography (S200, 16/300, GE Healthcare Life Sciences) in 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... After cell sonication proteins were affinity purified using a Glutathione sepharose 4b or remains on sepharose for pull down (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Molecular Biology 2020Quote: ... Immobilized Metal Affinity Chromatography (IMAC) was performed for protein purification using an AKTA Start Chromatography system (GE Healthcare Bio-Science, Sweden). The soluble fraction containing TNFα was diluted (1:1 ...
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bioRxiv - Microbiology 2019Quote: ... HSP40 fractions containing purified protein were pooled and further purified via anion exchange using a Mono Q anion exchange chromatography column (GE Healthcare) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: ... Analytical size exclusion chromatography using a Superdex 200 Increase 10/300 column confirmed the absence of aggregates from the protein preparation (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... A second purification step was performed by loading Protein A eluate on a Hiload 16/600 Superdex 200 pg gel filtration column (GE Healthcare), which was equilibrated and run using DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer C with 0.005% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...