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Citations for GE Life Sciences :
3551 - 3600 of 4376 citations for Recombinant Bovine CTLA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... and proteins were collected from culture supernatants and purified using the HisTrap HP column (GE Healthcare Life Sciences, Pittsburgh, PA) as described previously 33 ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... the labeled proteins were verified by running an SDS-PAGE and scanning using a Typhoon scanner (GE Healthcare, Danderyd, Sweden).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and proteins were transferred to Cytiva Amersham™ Protran™ 0.45 µm NC nitrocellulose blotting membranes (GE Healthcare, Munich, Germany). After blocking with 5% bovine serum albumin (Roth ...
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bioRxiv - Immunology 2024Quote: ... The complexes of SH-01AF594 with C3d and Fab 3E12 were obtained by concentrating ∼1 mg of either protein with 300µg of SH-01AF594 to ∼200µL before removing the excess protein via SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated and loaded onto a S75 16/600 size exclusion chromatography column (GE Healthcare, Chicago, USA). After chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... with the exception that the protein eluted from the Ni-NTA column was loaded on HiTrap Heparin column (GE Healthcare) equilibrated with HP1 buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... S9.6 antibodies were purified according to standard procedures (Stork et al., 2016) by using a HiTrap Protein G HP column (GE Healthcare), followed by extensive washing with 20 column volumes of PBS and elution with five column volumes of elution buffer (0.1 M glycine-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample was centrifuged for 10 minutes at 12 000 g and the supernatant kept to pulldown biotinylated proteins by adding 200 µL of streptavidin-coupled magnetic beads slurry (Streptavidin Mag Sepharose, GE Healthcare) previously washed and equilibrated ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was kept to pulldown biotinylated proteins by the addition of 100 µL of streptavidin-coupled magnetic beads slurry (Streptavidin Mag Sepharose, GE Healthcare) previously washed and equilibrated ...
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bioRxiv - Molecular Biology 2021Quote: ... The secreted proteins were purified from the harvested media by two-step chromatography – an IMAC was performed on a Talon column (GE Healthcare), followed by SEC on a Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The fractions containing the protein were pooled and applied onto a size-exclusion chromatography column (HiLoad 26/600 Superdex 200 pg, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequential affinity chromatography was performed on the combined fractions containing the desired asymmetric protein complexes from the size exclusion chromatography step by first using a Ni-sepharose HisTrap column (GE Healthcare) and then a StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the proteins were concentrated with Amicon Ultra 10K filter (viMillipore) and applied to HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions containing either AsCas12a or SpCas9 were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the heparin-purified protein was concentrated by ultrafiltration and applied to a size exclusion column (Superdex 200 16/600 column, GE Healthcare). The protein was eluted with Buffer C (20 mM HEPES ...
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bioRxiv - Plant Biology 2019Quote: ... The N-terminal 6xHis tag was cleaved with TEV protease and the protein further purified using a heparin (LUXMYB) or Superdex 200 (LUXFL) column (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...