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Citations for GE Life Sciences :
3551 - 3600 of 4746 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the membranes were incubated with an anti-rabbit peroxidase-conjugated secondary antibody (GE healthcare). Chemiluminescence was obtained by the ICL Substrate (GE healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to HRP required for western blot were obtained from GE Healthcare.
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies as well as ECL (enhanced chemiluminescence) reagents were obtained from GE Healthcare Life Sciences (Pittsburg ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 μl of a 1:1000 dilution of anti-rabbit HRP antibody (GE Healthcare) was added per well ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and incubated overnight with primary antibodies (aFLAG 1:2,500, aHSP90 1:50,000, GE Healthcare) containing 0.01% Sodium azide (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The membrane was dried and reprobed with an anti-mCherry antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected using HRP-conjugated secondary anti-mouse (GE Healthcare®, NA931V) and anti-rabbit (GE Healthcare® ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated anti-mouse and anti-rabbit secondary antibodies (Cell Signaling or GE Healthcare) were used at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were rabbit IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA) or mouse IgG HRP conjugated (GE Healthcare life sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... and detected through horseradish peroxidase conjugated α-rabbit antibodies (GE Healthcare Life Sciences, NA934V). Experiments were repeated independently three times ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then incubated overnight with anti-BrdU antibody (RPN202, GE Healthcare, Buckinghamshire, UK) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody was goat anti-rabbit IgG at 1:5000 dilution (GE Healthcare). Biotinylated proteins were blotted using HRP- conjugated Streptavidin at 1:1000 dilution (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HPR)-conjugated secondary antibody (GE Healthcare, USA) diluted 1 in 2500 in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (GE Healthcare) and rat anti-HA (Roche ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used: anti-mouse-IgG-Horseradish Peroxidase (GE Healthcare, Buckinghamshire, UK, 1:4000).
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bioRxiv - Cancer Biology 2022Quote: ... The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare). Human MSLN (Advance BioMatrix 5123-0.1mg ...
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bioRxiv - Plant Biology 2022Quote: ... antibodies at proper dilution and visualized with enhanced chemiluminescence (ECL) reagent (GE Healthcare, USA).
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with horseradish peroxidase-linked anti-rabbit IgG (GE Healthcare, UK), and ECL Prime Western Blotting Detection Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were detected using ECL Western Blotting Detection Reagent (GE Healthcare: #95038-562). Immunoblot band densitometry and normalization to GAPDH expression was performed using NIH ImageJ software35.
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bioRxiv - Cancer Biology 2023Quote: ... after primary and secondary antibody incubations and detected with ECL detection reagent (GE healthcare).
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bioRxiv - Neuroscience 2024Quote: The SOD specific antibodies were coupled to CNBr-activated Sepharose Fast Flow (GE Healthcare) following the manufacturer’s protocol at a concentration of 5 mg/mL wet gel ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Biophysics 2024Quote: ... we used horseradish peroxidase conjugated secondary antibodies against mouse (GE Healthcare, Cat. No.: NA931V) or rat (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... and sheep anti-Mouse IgG - Horseradish Peroxidase antibody (GE Healthcare, Cat # NA931; RRID: AB_772210).
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bioRxiv - Molecular Biology 2024Quote: ... Anti-GST primary antibody was used at 1:1,000 (GE Healthcare 27-4577-01) and secondary antibody was conjugate to Alexa 680 nm dye at 1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were HRP-linked donkey anti-mouse IgG (1:5000, GE Healthcare) and HRP-linked donkey anti-rabbit (1:5000 ...
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bioRxiv - Plant Biology 2024Quote: ... and ECL anti-mouse IgG horseradish peroxidase-linked whole antibody (1:1,000; GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... anti-rabbit or anti-goat secondary antibodies (0.5 μg/ml, GE Healthcare, Mississauga, ON). Blots were developed using a chemiluminescence reagent followed by analysis using the ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...