Labshake search
Citations for GE Life Sciences :
3551 - 3600 of 3996 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
-
bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The extracellular media was then diluted two-fold with 20 mM HEPES buffer at pH 7.0 and applied to 1 column volume (CV) of DEAE Sepharose Fast Flow Resin (from GE Healthcare) equilibrated with 10 CV of 20 mM HEPES buffer at pH 7.0 (1 CV equaled 2 ml of settled resin for 25 ml cultures and 1 CV equaled 4 ml of settled resin for 250 ml cultures) ...
-
bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
-
bioRxiv - Bioengineering 2020Quote: ... and supernatant was filtered with a 0.22 μm filter before loading onto a 1 ml HisTrap HP column (GE Healthcare). Proteins bound to the column were washed with 10 column volumes of washing buffer (50 mM Tris ...
-
bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... and transferred by western blotting (30V, 1 hour) to polyvinylidene fluoride membrane (P 0.45 μm; 10600023 Amersham Hybond, GE Healthcare). Blots were probed with biotinylated GNA lectin (40 μg/ml ...
-
bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
-
bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
-
bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes were soaked with 1:5000-diluted HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) in TBST containing 5% skim milk for 30 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... reverse-crosslinked samples were combined and the DNA was purified using solid phase reverse immobilization (SPRI) Sera-Mag SpeedBeads (1.6:1 ratio, GE Healthcare). The final library was eluted with 22 μL ddH2O.
-
bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... A second purification step was performed by size-exclusion chromatography (SEC) with Superdex 75 column (1 x 30) (GE Healthcare) at a flow rate of 0.5 mL/min monitored at 280 nm using a 25 mM Na2HPO4/NaH2PO4 ...
-
bioRxiv - Microbiology 2020Quote: ... Lysates were clarified and the supernatant filtered through 0.22 μm filters and subjected to nickel affinity chromatography using 1 ml HisTrap HP columns (GE Healthcare). Elution was carried out via a 25-500 mM imidazole gradient in 50 mM Tris [pH 8] ...
-
bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
-
bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mouse LRRC23 (residue 171-340 aa, CCDS20528.1) was cloned and inserted into pGEX6p-1 expression vector (GE healthcare), followed by transformation into E ...
-
bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
-
bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
-
bioRxiv - Biophysics 2022Quote: ... at a 1:3 protein:dye molar ratio for 1 h at room temperature and then desalted on a PD-10 desalting column (GE Healthcare), where the free dye and the conjugated Alexa488-αSOs were separated ...
-
bioRxiv - Cancer Biology 2022Quote: ... phospho-p38 MAPK (Invitrogen, #36-8500, 1:1000. Chemiluminescence was recorded using the Image Quant Las4000 imaging system (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... Images were acquired as 3D stacks using a 100X lens in a Delta Vision Deconvolution system equipped with an Olympus 1×70 microscope and images were deconvolved using SoftWoRx 3.0 (Applied Precision) and mounted using Photoshop.
-
bioRxiv - Molecular Biology 2022Quote: ... After passing through a 0.4 μm filter the supernatant was loaded onto a HisTrap HP 1 mL column (GE Healthcare). The protein was eluted with a lysis buffer supplemented with 300 mM Imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... coli supernatant was directly applied onto a 1-ml Ni2+ ion chelating poly-histidine affinity column (HisTrap HP, GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... The supernatant was isolated by centrifugation at 32,000 rpm for 1 h and incubated with Ni-NTA beads (GE Healthcare) for 1.5 h at 4 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
-
bioRxiv - Plant Biology 2022Quote: ... Additional purification was carried out by anion-exchange chromatography on a 1 mL HiTrap Q Sepharose column (GE Healthcare, USA). Yields of the recombinant-produced proenzymes ranged from 0.1 to 2 mg per litre culture ...
-
bioRxiv - Pathology 2022Quote: ... High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare, UK) for RNA hybridization in membranes ...
-
bioRxiv - Plant Biology 2022Quote: ... Acetonenitrile was then added to 70 % and a 10:1 ratio of magnetic beads (Hydrophilic-Part no: 45152105050250, GE Healthcare plus Hydrophobic-Part no ...
-
bioRxiv - Neuroscience 2022Quote: ... were applied for 1 h at room temperature and detected with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences) in Fusion FX7 image and analytics system (Vilber Lourmat).
-
bioRxiv - Plant Biology 2024Quote: ... NATA2Δ) as well as the full-length NATA2 from Arabidopsis were cloned to the pGEX6P-1 expression vector (GE Healthcare). The plasmids were then transformed into Rosetta 2 (DE3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
-
bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered (0.45 polyvinylidene fluoride membrane, Carl Roth, Germany) and applied onto a 1 ml Nickel-IDA column (GE Healthcare) equilibrated in 50 mM TRIS-HCl pH 7.8 ...
-
bioRxiv - Biochemistry 2024Quote: ... the independently purified SMC1ACC-EQ/RAD21C and SMC3CC-EQ/RAD21N samples were mixed with a 1:0.9 ratio and loaded onto a Superdex 200 16/600 column (GE Healthcare) equilibrated with the purification buffer (200 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
-
bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: His-SUMO-FacZ127-145 and GpsB1-75 were thawed from frozen aliquots and separately buffer-exchanged into an identical buffer (20 mM HEPES, pH 7.6, 500 mM NaCl, 1 mM DTT) using PD10 de-salting columns (GE Healthcare). Gel filtration samples were made by mixing GpsB1-75 at 90 μM with SUMO-FacZ127-145 at 30 μM in 500 μL reactions ...
-
bioRxiv - Microbiology 2023Quote: The supernatant was filtered with a 0.22 μm filter and was loaded onto a pre-equilibrated HisTrap HP 1 ml column (GE Healthcare) at 0.5 ml/min with a peristaltic pump at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... vivax SAL-1 antigen was prepared from Aotus iRBCs purified by Percoll™(GE Healthcare Bio-Sciences AB, Uppsala, Sweden) cushion (47% ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 8 ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 7 ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...