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Citations for GE Life Sciences :
301 - 350 of 5121 citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... previously calibrated with protein standards (GE Healthcare), including thyroglobulin (669 kDa) ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hrs at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies (antibodies listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... or agarose Protein G beads (GE Healthcare) only ...
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bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
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bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Protein A-Sepharose CL-4B (GE Healthcare) was incubated with cell lysates and polyclonal anti-IqgC serum [55] ...
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Purified IgGs were tested for binding to OVA in ELISA and in an SPR assay using a BIAcore T200 apparatus (GE Life Sciences, Piscataway, NJ) in a protein A capture format ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).