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Citations for GE Life Sciences :
301 - 350 of 998 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
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bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
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bioRxiv - Biophysics 2024Quote: ... N-myc proteins were polished using size-exclusion chromatography (SEC) using a Superdex 16/600 S75 column (GE Healthcare). GB1-tagged N-myc proteins were purified in a similar manner with a few alterations ...
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bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
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bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
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bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... 100 pmoles of DNA samples were blotted on a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare Life Science). The DNA was then UV crosslinked to the membrane (254 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μg of total RNA from each sample was analysed through 1.2% agarose-formaldehyde gels and transferred to HybondTM-N+ membrane (GE Healthcare) by neutral transfer ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were separated on 0.8% agarose gel and transferred by capillarity to the Amersham Hybond N+ membrane (GE Healthcare). DNA hybridization was performed with the Amersham AlkPhos Direct Hybridization Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Small RNAs were separated on a 15% sequencing gel and transferred to Amersham Hybond-N+ membranes (GE Healthcare, cat# RPN303B) using a Trans-blot SD semi-dry electrophoretic transfer cell (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Gels were washed for 15 min in 0.25 N HCl before an overnight transfer to a charged nylon membrane (Hybond-XL, GE Healthcare) in 0.4 N NaOH ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Pathology 2022Quote: ... High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare, UK) for RNA hybridization in membranes ...
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bioRxiv - Plant Biology 2022Quote: ... HinfI-restricted genomic DNA was separated on a 1.2 % agarose gel and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Microbiology 2023Quote: ... The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+, GE Healthcare, #RPN303B) was done as via capillary transfer of RNA from the denaturing agarose gel to the nylon membrane (Sambrook ...
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bioRxiv - Genetics 2024Quote: ... [ptch+] were separated on a 1% agarose gel in 1X TAE supplemented with 6.66% formaldehyde and transferred to a positively charged nylon membrane (Hybond N+, GE Healthcare) using capillary transfer ...
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bioRxiv - Microbiology 2024Quote: RNA was separated on a 1.5% formaldehyde-agarose gel and RNA transfer to a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare) occurred overnight using a capillary blot system ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of gDNA (containing indicated amounts of DNA) was denatured with 0.5 N NaOH for 15 minutes then spotted on a nitrocellulose blotting membrane (GE Healthcare) and cross-linked at 120,000 microjoules for 20 min using a Stratalinker® UV Crosslinker 1800 (Stratagene) ...
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bioRxiv - Immunology 2021Quote: The affinity measurements of hemin towards wild type (hCD36ecto) or mutants (R292A, D372A and Q382A) were performed in MicroCal iTC200 isothermal titration calorimeter (GE Healthcare, Chicago, USA). Unless specified ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 and 200 ng of DNA from each fraction and from unfractionated genomic DNA were blotted on a membrane (Amersham Hybond -N+, GE Healthcare) using a BioDot apparatus (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The RNA lanes were blotted through classic upward blot onto a Amersham Hybond N+ membrane (GE Healthcare Life Science, Little Chalfont) by neutral transfer (20× SSC ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... purified RNA was electrophoresed in non-denaturing 2% agarose TBE gel and then gel was blotted onto an Hybound-N nylon membrane (RPN303N, GE Healthcare) by semidry electroblotting in 0.5× TBE buffer for 2 hours at 200mA ...
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bioRxiv - Cell Biology 2021Quote: ... equipped with an Olympus UPIanApo 100x numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further subjected to SEC (Superdex 200 column for KASH5-NCC and LIC and Superdex 75 column for KASH5-N, GE Healthcare; buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with an Olympus UPIanApo 100× numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...