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Citations for GE Life Sciences :
301 - 350 of 770 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cultures were collected on Whatman GF/C filters with 1.2 μm pore size (GE Healthcare, US) under vacuum (750 mbar) ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: SPR experiments were carried out at 25 °C on a Biacore 8K SPR system (GE Healthcare). Series S SA sensor chips (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™, GE Healthcare) in plates containing ZYP agar medium (supplemented with 100 µg.mL-1 ampicillin and 34 µg.mL-1 chloramphenicol) ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare, Mississauga, ON) in the dark at room temperature for 30 min followed by quenching with 15 mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... time-lapse movies of high resolution were recorded at 37 °C on a DeltaVision Elite (GE Healthcare) widefield microscope using a 60×/1.42 NA oil objective and a step size of 0.25-0.35 μm (Figure 1A-C ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Biophysics 2022Quote: SPR analysis were carried out at 25.0 °C using Biacore T100 system (GE Healthcare, Chicago, IL, USA). In all protocols ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were used for silverstaining (PageSilver kit, Fermentas) or transfer to Hybond-C nitrocellulose mebranes (GE Healthcare). After overnight blocking in PBS with 5% nonfat dry milk and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was filtered (1.2 μm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom), acidified to pH ≤ 1 for sterilization using 30% hydrochloric acid ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biophysics 2022Quote: ITC experiments were performed at 25°C by using a MicroCal VP titration calorimeter (MicroCal-GE Healthcare) under the same conditions as described previously (16) ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Molecular Biology 2020Quote: All the surface preparation experiments were performed at 25°C using a Biacore 3000 instrument (GE Healthcare). Purified Rubisco was immobilized on CM5 sensor chips using a Biacore Amine Coupling Kit according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Separation was performed at 20°C using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with a flow rate of 0.5 mL min−1 and a mobile phase consisting of 20 mM Na-HEPES pH 7.8 ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The purified rh proSP-C BRICHOS variants were analysed by Superdex 200 GL columns (GE Healthcare, UK) using an ÄKTA system (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C overnight and then scanned using an Amersham Typhoon scanner (GE Healthcare). Individual infected cells were quantified using ImageQuant TL software (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... To isolate activated protein C (aPC) ion exchange chromatography with FPLC (ÄKTAFPLC®, GE Healthcare Life Sciences) was used ...
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bioRxiv - Plant Biology 2022Quote: ... Gels were dried at 80 °C for 2 hrs and covered with phosphor imaging plate (GE Healthcare) overnight after RNA fixation in RNA fixation buffer (40% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Soluble PB2 C-terminal domains were purified by affinity chromatography on HisTrap columns (GE Healthcare Life Sciences) and analyzed by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... +4°C) and supernatant was loaded into a 5 mL Nickel sepharose column (HisTrap HP, GE Healthcare). The loaded column was first washed ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... These were stored in a chilled compartment at 9°C within a skirted 384 well plate (GE Healthcare) before being washed over the chip surface in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: All SPR measurements were performed at 25° C using a Biacore 3000 instrument (BIAcore, GE Healthcare, Piscataway, NJ) as described previous24,25 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...