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Citations for GE Life Sciences :
301 - 350 of 874 citations for Cytokeratin 8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... adjusted to pH 8 with HCl) and applied to an HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) to further separate the low molecular weight contaminants and obtain purified trimers (ÄKTA FPLC, GE Healthcare). Eluted trimer fractions were assessed on the SDS-PAGE gel to determine the purity and selected fractions were pooled and passed through 0.22 µm filter to sterilize the sample ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Genomics 2020Quote: ... Mouse Anti-Rabbit IgG (Light Chain Specific) (CST, #93702S) (1:5000) or Rabbit IgG HRP Linked Whole (GE Healthcare, NA9340), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... 0.012% LMNG was concentrated up to 8 mg/ml using a 30 kDa MWCO Vivaspin 20 concentrator (GE healthcare). Extensive optimisation of crystal conditions using sitting-drop and hanging-drop vapour diffusion and other post-crystallisation treatments yielded plate shaped crystals after 3-4 days of incubation at 20°C ...
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bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-anti rabbit secondary (GE Healthcare; 1:5000) and HRP-anti mouse secondary (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-rabbit (NA934, GE Healthcare, 1:10000) and sheep anti-mouse (NA931 ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were anti-rabbit (NA934V, GE Healthcare) or anti-mouse (NA931V ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-rabbit IgG (sheep polyclonal, NA934: GE Healthcare), and anti-alpaca IgG65 (goat polyclonal ...
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bioRxiv - Genomics 2019Quote: ... Conjugated secondary antibodies against rabbit (GE Healthcare NA934) or guinea pig (Sigma AP108P ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rabbit IgG from donkey (GE Healthcare, NA934); Alexa Fluor 488 chicken anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2020Quote: ... α –EDS1 (Rabbit) and horseradish peroxidase-coupled (GE Healthcare) or alkaline phosphatase-coupled (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and donkey anti-rabbit (GE healthcare; 1:5000).
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-rabbit (#NA9340V, GE Healthcare, 1:4000). Membranes were washed after each incubation step three times for 5 min in TBS-T buffer (25 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-rabbit (NA9340; GE Healthcare; 1:25,000). Protein extraction was performed using lysis Buffer (20 mM Tris (pH 8) ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibodies were anti-rabbit IgG (GE Healthcare) and anti-mouse IgG (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit secondary antibody (GE healthcare,1:10000). Membranes were developed by the Odyssey Fc Imaging system (LI-COR Bioscience).
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bioRxiv - Molecular Biology 2020Quote: ... HRP-linked anti-Rabbit IgG (NA934, GE Healthcare) and HRP-linked anti-Mouse IgG (NA931 ...
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bioRxiv - Molecular Biology 2023Quote: ... or α rabbit (GE Healthcare, Life Sciences NA934V) HRP conjugated antibodies were used ...
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bioRxiv - Plant Biology 2022Quote: ... and an-rabbit-HRP secondary antibody (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Peak TopBP1b6-8 fractions (eluted with 50mM and 75mM imidazole) were desalted (HiTrap™ or HiPrep 26/10, GE Healthcare) in physiological buffer (10mM Na2H/NaH2 PO4 pH 7.6 ...
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bioRxiv - Genomics 2020Quote: ... Equivalent amounts of protein were resolved by 8-10% SDS-PAGE and blotted onto nitrocellulose (0.2 µm, Amersham Protran) with a semi-dry blotter (GE Healthcare). The membrane was stained with Ponceau S solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... T1-weighted anatomical MRI and diffusion-MRI scans were collected from 11 out of the 13 healthy individuals (8 male, 35.2 +/- 12.25 years) on a 3.0 Tesla General Electric Signa Excite HDx (GE Healthcare, Waukesha, WI) clinical MRI system with an eight-channel head receive-only coil ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Genetics 2020Quote: Floxed Rasa3 exon three was excised in adult (8-12 weeks of age) hematopoietic cells by inducing Mx1-Cre recombinase activity with Poly(I:C) (GE Healthcare). Mice carrying floxed Rasa3 allele(s ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were electrophoretically separated on an 8% polyacrylamide gel and then wet- transferred to a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare). Prior to antibody incubation ...
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bioRxiv - Bioengineering 2021Quote: ... The UtAs were scanned using the C4-8 transabdominal probe of a GE Voluson E10 (GE Healthcare, Wisconsin, United States) US machine ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...