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Citations for GE Life Sciences :
301 - 350 of 1516 citations for Alpha 2 HS glycoprotein Fetuin A Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant containing cDNA-linker-VHH complex was purified using His Mag Sepharose Ni beads (GE Healthcare, USA). The supernatant was added to 20 μL of the beads and incubated at 25°C for 30 min ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and the His-tagged sec49K was purified with HisTrap excel Ni beads (GE Healthcare), eluted with 300 mM imidazole and dialysed against buffer containing 50% glycerol (50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag was separated from DBD on an S75 10/300 GE gel filtration column (GE healthcare) equilibrated with 10 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on an affinity chromatography column (GE Healthcare, His-Trap FF crude, #17-5286-01) with a flow rate of 1 mL/ min ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Immunology 2022Quote: ... and HEK293 cell lines were obtained from Royan Institute Cell Bank and cultured in Dulbecco’s Modified Eagle Medium (DMEM, GE Healthcare Life Sciences, Pittsburgh, PA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL, GE Healthcare). The protein was digested with PNGase F (New England BioLabs ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were filtered (0.45 μm) to remove residual cellular debris and loaded onto a His-Trap Column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The concentrated sample was further purified over a Superdex 200 Hi-load 16/60 gel filtration column (GE Healthcare) pre-equilibrated with 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: The interaction between PttP1 and PttP2 was studied by a pulldown assay using His SpinTrap™ columns (GE Healthcare) with a bed volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: ... tag cleavage and dialysis followed by gel filtration on a Superdex 75 Hi Load column 16/60 (GE Healthcare) pre-equilibrated with 20mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... and loaded to a Hi-Load 16/600 Superdex-200 pg (preparation grade) size-exclusion chromatography column (GE Healthcare) equilibrated with the gel filtration buffer (100 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... His-2xFYVE-containing fractions were pooled and subjected to size exclusion chromatography using a Superdex 75 column (GE HealthCare). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Both untagged and His-tagged Impβ were further purified using a Superdex 200 16/60 gel filtration column (GE Healthcare) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... The recovered supernatant was filtered using 0.45 μm syringe-driven filters (Jet Biofil) and loaded in a His-Trap column (GE Healthcare) equilibrated with 350 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was concentrated on an Amicon Ultra Centrifugal Filter Unit (10 kDa cut-off) and injected at 0.5 ml/min on a Hi-Load 16/60 Superdex column (GE healthcare), equilibrated with 20 mM Tris-HCl buffer ...
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bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
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bioRxiv - Biochemistry 2021Quote: ... XLF-6xHis was purified using a HP His-trap column followed by a HiTrap Q HP column (both GE Healthcare). mSUMO1 (#UL-712) ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... His-CD147 (produced by our laboratory) was fixed to the surface of CM5 sensor chips (GE Healthcare Bio-Sciences AB) by amino coupling kit (GE Healthcare ...