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Citations for GE Life Sciences :
301 - 350 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged using Typhoon 5 Imager (GE Healthcare) to record signals from the Cy5 dye ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare) was used for further purification which resulted in a monodisperse and clear INO80 complex ...
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bioRxiv - Biophysics 2019Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and finally eluted with 220 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... on a MonoQ 5/50 GL column (GE Healthcare). Sample purification and analysis with HPLC were performed using Agilent 1260 Infinity (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl of streptavidin beads (GE Healthcare, Chicago, USA) per O.D.254 unit was added ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... A HisTrap HP column (5 ml, GE Healthcare, France), prepacked with Ni High Performance Sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were scanned using a Typhoon 5 (GE Healthcare) and the signal intensity quantified using Image Studio Lite (LI-COR Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... applied to a 5 ml HisTrap HP (GE Healthcare), and washed with lysis buffer using an Äkta pure fast-performance liquid chromatography system (GE Healthcare,) ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare, Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using a 5 mL HisTrap FF column (GE Healthcare). Bacterial pellets were lysed in a buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... using a SD200 Increase 5/150 column (GE Healthcare) at 0.4 ml/min with 1 frame recorded per second (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... first with HisTrap HP 5 mL columns (GE Healthcare) and next with a size-exclusion HiLoad Superdex 200 PG column (GE healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting sample was applied to a 5 ml HiTrap HP heparin column (GE Healthcare) equilibrated with TGE containing 50 mM NaCl and 1 mM β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and eluted with imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % glycerol on an ÄKTA Pure (GE Life Sciences). Where nucleotide was present ...
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bioRxiv - Molecular Biology 2023Quote: ... Heparin columns (5 ml) were purchased from GE HealthCare or Cytiva.
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bioRxiv - Microbiology 2023Quote: ... The Mono Q 5/50 GL column (GE Healthcare) was equilibrated with 50 mM Bicine buffer (pH 8.5 ...
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bioRxiv - Biochemistry 2024Quote: ... loaded onto 5 ml GST-trap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Systems Biology 2019Quote: ... 0.7% (v/v) acetonitrile (ACN) and 1% (v/v) carrier ampholytes mixture (IPG buffer pH 3.0-10.0, GE Healthcare). Fractionation was performed on a 3100 OFFGEL (OGE ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2019Quote: ... 5% glycerol) to a final NaCl concentration of 20 mM and immediately loaded on a 5 mL HiTrapQ column (GE Healthcare Life Sciences) as a second purification step ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The dissolved ammonium sulfate pellet was loaded on a 5 mL DEAE Sepharose and 5 ml Ni-Sepharose cartridge (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2023Quote: ... Active fractions were combined and exchanged into buffer B (50 mM Tris-HCl, pH 7.5) before being applied to a Mono Q™ HR 5/5 column (GE Healthcare, Piscataway, NJ, USA) equilibrated with the same buffer ...