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Citations for GE Life Sciences :
301 - 350 of 360 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was purified on a 5ml nickel nitrilotriacetic acid (Ni2+-NTA) resin (GE Healthcare). The column was washed stepwise with 20 ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then applied to Ni2+-NTA (Nickel-Nitrilotriacetic acid) sepharose column (GE Healthcare, USA) pre-equilibrated with the lysis buffer at a flow rate of 0.2 ml/min ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid (400 ng) was spotted on to Amersham Hybond N+ nylon membrane (GE Healthcare Lifesciences), air-dried and UV cross-linked for 3 min using a transilluminator ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) trifluoroacetic acid and applied to a Superdex 30 3.2/300 column (GE Healthcare). Fractions containing crosslinked peptides were evaporated to dryness ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Cell Biology 2022Quote: ... URA3: CaUra3pXhoI and CaURA3m81) and Amersham ECL Direct Nucleic Acid Labelling and Detection System kit (GE Healthcare UK Limited ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was loaded onto a 5 mL nickel-nitrilotriacetic acid (Ni-NTA) resin (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
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bioRxiv - Immunology 2020Quote: ... acid-eluted immunoprecipitates were separated by SDS-PAGE under reducing conditions and transferred to HybondTM ECLTM membranes (GE Healthcare). Membranes were incubated sequentially with the different primary antibodies and developed with the appropriate HRP-conjugated secondary antibody using enhanced chemiluminescence (ECL) ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
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bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid ) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
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bioRxiv - Neuroscience 2024Quote: The binding kinetics of 5G2 and 1G11 to tau peptides encompassing amino acids 407-421 and 407-423 were undertaken through Biacore 2000 (GE Healthcare) using surface plasmon resonance analysis according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... FcγRⅠ WT was diluted in acetic acid buffer pH 5 and immobilized on the surface of Series Sensor Chip CM5 (GE Healthcare) by amine coupling method ...
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bioRxiv - Biochemistry 2024Quote: WT ubiquitin was expressed in a tagless form and purified with the glacial acetic acid method followed by gravity cation exchange chromatography (GE Healthcare) and SEC as described previously.77
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...