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Citations for GE Life Sciences :
301 - 350 of 2233 citations for 3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Images were taken at 2 second intervals for 1 minute and deconvoluted with Applied Precision softWorx imaging software (GE Healthcare). The microtubule comet tracking and quantification was performed with the MTrackJ plugin in ImageJ/Fiji (Meijering et al. ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2 x 1 mL or 1x 5 mL HiTrap Q HP, GE Healthcare) equilibrated with Q_A buffer ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM L-Glutamine (GE Life Sciences #SH30852.01), 100 units/mL Penicillin and 100 μg/mL Streptomycin (Corning #30-001-CI ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM βME using Glutathione Sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Cancer Biology 2022Quote: ... siRNA non-targeting pool #2 (GE Healthcare Dharmacon) was used as a control for all the knockdown experiments ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... (2) Ni Sepharose fast flow resin (GE Healthcare) chromatography using a step elution from 50 mM to 200 mM Imidazol in Tris buffer B (20 mM Tris/HCl ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare, Vélizy-Villacoublay, France), 0.5% penicillin-streptomycin (Invitrogen)) ...