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Citations for GE Life Sciences :
3401 - 3450 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was further purified by size exclusion chromatography (SEC) using Superose 6 Increase 10/300 column (GE Healthcare), pre-equilibrated with the desired buffers.
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bioRxiv - Biophysics 2022Quote: ... Protein was further purified by size exclusion chromatography using a Superose 6 Increase column (10/300 GL, GE Healthcare) equilibrated with 150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized with the LAS-4000 system (ImageQuantTM LAS-4000, GE Healthcare Life Sciences, Pittsburgh, PA, USA) after a chemiluminescent reaction (Immobilon® Western Chemiluminescent HRP Substrate ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Immunology 2022Quote: ... the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The eluted protein was concentrated and purified by gel-filtration chromatography (Superdex 200 10/300 GL, GE Healthcare, USA), using a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The cleaved protein was collected and further purified using gel filtration (HiLoad 16/60 Superdex pg 200, GE Healthcare) with GF buffer (20 mM Tris-HCl ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... Lipoproteins were fractionated using a Superose 6 10/300 GL fast protein liquid chromatography (FPLC) column (GE healthcare, UK). Fractions (500 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 column (Cytiva, formerly GE Life Sciences) in buffer B ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified via size exclusion chromatography using a Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... Purification was carried out following manufacturer’s instructions on HiTrap Protein G HP purification column for IgG mAbs (GE Healthcare), and a POROS CaptureSelect IgM-XL Affinity Matrix Column (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... Protein was purified as previously described [59] but with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) for size exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta pure system (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Refolded HLA-G2 protein was purified by size exclusion chromatography using HiLoad 26/60 Superdex75 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Final purification was performed by loading the concentrated protein onto a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted Clr4 proteins were further purified by a Superdex 75 10/300 GL size-exclusion column (GE Healthcare) pre-equilibrated in size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein A/G Sepharose beads (17-5280-01, 17-0618-01 - GE Healthcare (3:1 ratio A/G beads)) were added for 2 hours to precipitate antibody bound complexes ...
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bioRxiv - Microbiology 2024Quote: ... We then purified and concentrated S2635 and S2782 using Protein A-MabSelect SuRe or PrismA affinity columns (GE HealthCare) as described(73) ...
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bioRxiv - Immunology 2024Quote: ... the SOSIP-component A fusion protein (329 SOSIP.v8.2-I53-50A.1NT1) was passed through a Superose 6 Increase 10/300 GL (GE Healthcare) SEC column in Assembly Buffer II (25 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... Individual pooled target proteins were further purified by passage through a Hiload 16/60 Superdex 200 column (GE Healthcare) with buffer E (150 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... unpurified proteins contained in HEK293F filtered supernatants were captured on PGT145-functionalized CNBr-activated sepharose 4B beads (GE Healthcare) by overnight rolling incubation at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 1997) were purified from the cell culture supernatants by affinity chromatography using Protein A-MabSelect SuRe (GE Healthcare, USA). Purity was assessed by SDS-PAGE under both non-reducing and reducing conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were incubated with 30 µL of protein G Sepharose™ 4 Fast Flow (#17-0618-01, GE Healthcare) under agitation for 4 hours at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Deglycosylated proteins were purified to homogeneity by size exclusion chromatography using a HiLoad Superdex 75 PG column (GE Healthcare) equilibrated in Dulbecco’s Phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... was used to visualize proteins on an Amersham Imager 600 RGB gel and membrane imager (GE Life Sciences/Cytiva).
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to 0.45 µm pore-size nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked at room temperature for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were separated by Size Exclusion Chromatography with HiLoad® 16/600 Superdex® 75 pg (GE HealthCare), which were previously equilibrated in 50 mM NaPi pH 7.0 ...
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bioRxiv - Biophysics 2024Quote: ... N-myc proteins were polished using size-exclusion chromatography (SEC) using a Superdex 16/600 S75 column (GE Healthcare). GB1-tagged N-myc proteins were purified in a similar manner with a few alterations ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA binding was measured by capturing protein-RNA complexes on Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) and unbound RNA on a Hybond-XL (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The GST fusion protein was purified from the soluble extract by affinity chromatography using Glutathione Sepharose 4B (GE Healthcare). The GST-tag was cleaved off using thrombin ...
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bioRxiv - Genomics 2024Quote: ... GST-tagged bat APOBEC proteins were purified using Glutathione Sepharose High-Performance beads (GE Healthcare, Cat # 17-5279-01) as previously described30 ...
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bioRxiv - Biochemistry 2024Quote: ... size exclusion chromatography was performed by injecting the concentrated protein into a HiLoad 16/60 S200 column (GE Healthcare) equilibrated with buffer (25 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2024Quote: ... The concentrated protein was further purified by size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) equilibrated with SEC buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was further purified through SEC using a Superdex™ 75 Increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... that recognizes amino acids 3-8 of Aβ and the Protein G Sepharose 4 Fast Flow resin (GE healthcare) (Tables 2 and 3 ...
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bioRxiv - Biochemistry 2024Quote: ... The protein dye mixture was then desalted in a pre-packed Sephadex G-25M column (PD-10, GE Healthcare) using labeling buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The Elution Buffer was exchanged to Imidazole Buffer by passing the proteins through a PD-10 column (GE Healthcare) and for the His-cleaved samples incubated overnight at 10 °C with His-TEV protease ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by 10 or 12% SDS-PAGE and transferred to a 0.2-µm nitrocellulose membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in TBS-T (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... A second protein purification step was performed using a cation-exchange column (HiTrap SP HP, 5 ml, GE Healthcare) with a high salt elution buffer (50 mM Tris–HCl at pH 7.5 ...