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Citations for GE Life Sciences :
3351 - 3400 of 5289 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2 x 1 mL or 1x 5 mL HiTrap Q HP, GE Healthcare) equilibrated with Q_A buffer ...
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bioRxiv - Immunology 2024Quote: ... supernatants underwent protein affinity purification using a high-performance 5 mL HiTrap MabSelect SuRe column (Cytiva, catalog no. 11003494) from GE Healthcare Life Sciences for large-scale production ...
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bioRxiv - Biochemistry 2024Quote: ... The proteases were then loaded onto an equilibrated MonoS 5/50 GL ion exchange column (GE Healthcare Cat. #17-5168-01) and washed with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was cleared by centrifugation and loaded onto two serially connected HisTrap 5 ml columns (Cytiva) which were connected to a ÄKTA FPLC system (GE Healthcare). Columns were washed with lysis buffer and bound proteins were eluted with a linear imidazole gradient (20 – 500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... DsbA-RbmB was purified from the cleared lysate by loading the supernatant onto a cobalt-charged 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... Dried gels were then exposed to a storage phosphor screen for six days before acquiring radiograms using a Typhoon 5 Imager (GE Healthcare). All experiments were performed in three independent replicates that were carried out on three separate days ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered through a 0.45 µm membrane and loaded onto a nickel affinity column (5 mL GE Healthcare HisTrap). The protein was eluted using a gradient from 20 to 500 mM imidazole (solution B ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was separated from MBP using cation-exchange chromatography with a 5 ml SP Sepharose HiTrap column (GE Life Sciences). Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15 ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were loaded using a sample pump or super-loop (Cytiva) on a 5 mL Strep-Trap HP column (GE Healthcare) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... and buffer exchanged to PBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare). Protein concentrations were determined by Nanodrop using the proteins peptidic molecular weight and extinction coefficient as determined by the online ExPASy software (ProtParam) ...
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bioRxiv - Genetics 2020Quote: ... The telomeric extension products were separated by 10% TBE-PAGE and visualized by a PhosphorImager (GE Healthcare). TRAP products intensity in each lane were quantified by the ImageQuant Software and normalized to the respective internal control intensity.
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bioRxiv - Biochemistry 2021Quote: ... and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) in 50 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... 10 or 12 % SDS-PAGE and transferred and immobilized in a n.itrocellulose membrane (GE Healthcare, Munich, Germany) by semi-dry blotting ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing GRK5 were combined and run on a Superdex 200 10/300 GL column (GE healthcare). GRK5 was aliquoted ...
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bioRxiv - Molecular Biology 2020Quote: ... An Agilent BioInert HLPC/FPLC system equipped with Superdex 200 Increase 10/300 GL column (GE Healthcare) was used for the SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of protein was injected onto a Superdex 75 increase 10/300 GL column (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated and subjected to size exclusion chromatography on a Superdex-200 10/300 GL column (GE Healthcare) pre-equilibrated with either SEC100 buffer [100 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare). Fluorescence labeling was performed as previously described.32 In brief ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was transferred to appropriate buffers by size exclusion chromatography (Superdex 75 10/300, GE Healthcare) and stored at 4°C.
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bioRxiv - Microbiology 2020Quote: ... HR1-L6-HR2 protein was concentrated and gel-filtered on a 10/300 Superdex 75 (GE Healthcare) column ...
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bioRxiv - Immunology 2021Quote: ... Various concentrations of the RNA sensors diluted in running buffer (10 × HBS-EP+; GE Healthcare, Cat# BR100669) were injected as analytes at a flow rate of 30 μl/min ...
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bioRxiv - Biochemistry 2020Quote: ... The complexes were purified using SEC on a Superose™ 6 Increase 10/300 GL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT prior to injection onto a Superdex 200 10/300 GL column (GE Healthcare). PTEN and P-Rex2 alone at corresponding concentrations demonstrate the peak-shift upon complex formation.
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bioRxiv - Genomics 2020Quote: ... Eluted protein samples were further purified by gel filtration using Superose 6 10/300 column (GE Healthcare). These proteins were buffer-exchanged to storage buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... The WUS-HD was injected on a Superdex 75 10/300 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM TRIS/HCl (pH 8.0) ...
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bioRxiv - Biophysics 2020Quote: ... Protein samples were freshly prepared using size exclusion chromatography (GE Life Sciences, Superdex 75 10/300 GL) in a buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
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bioRxiv - Immunology 2021Quote: ... Further purification was achieved by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in buffer containing 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified by size-exclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column.
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bioRxiv - Biochemistry 2021Quote: ... The protein sample (50 μL) was loaded into a 10/300 Superdex® 200 GL (GE Healthcare) column connected to the MALS detection unit for SEC ...
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bioRxiv - Biochemistry 2020Quote: ... and purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer C (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and applied to size-exclusion chromatography with a Superdex 200 10/300 gel-filtration column (GE Healthcare) preequilibrated with buffer C (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 10 mM imidazole and incubated with Ni-Sepharose excel resin (GE Healthcare) for 2 that 4 °C ...
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bioRxiv - Biochemistry 2020Quote: Analytical size-exclusion chromatography was done with a Superdex 75 10/300 GL (GE Healthcare Life Sciences) on an ÄKTApurifier system (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biophysics 2021Quote: ... adjusted to 300 mM NaCl and purified on a Superose 6 Increase 10/300 GL (GE Healthcare) using buffer A containing 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... except containing 80 mM NaCl and no imidazole) using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Biophysics 2021Quote: ... Eluent was concentrated and loaded onto Superose 6 increase 10/300 GL size exclusion column (GE Healthcare) in buffer C (20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The eluate was further purified with Superdex Increase 200 10/300 GL size-exclusion column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... One milliliter of supernatant was loaded onto a gel filtration column (10 mm × 30 cm; GE Healthcare) and eluted with PBS at a rate of 0.5 ml/min on an AKTA Explorer 100 FPLC Workstation (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstituted protein was further purified on the Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in a buffer comprising 20 mM Bis-Tris pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... The SEC runs were performed using a Superose™ 6 increase 10/300 column (GE Healthcare/Cytiva) equilibrated in 0.025 M Tris-Base ...
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bioRxiv - Microbiology 2020Quote: ... sortase and excess DBCO-amine was removed using Ni-NTA resin and PD-10 columns (GE Healthcare) respectively ...
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bioRxiv - Microbiology 2021Quote: ... Gels were imaged for 10 minutes with increments of 30 seconds on the LAS4000 Imagequant (GE Healthcare) for in-gel Cy5 fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance profiles at 260 nm were determined during gradient fractionation using an ÄKTA Purifier 10 (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then loaded on SEC column (Superdex 200 Increase 10/300 GL column, GE healthcare) pre-equilibrated with Buffer B ...