Labshake search
Citations for GE Life Sciences :
3301 - 3350 of 3780 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: Dimer formation of non-farnesylated GBP1 was analyzed by analytical size-exclusion chromatography (Superdex 200 5/150, GE Healthcare) as described (Ince et al ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...
-
bioRxiv - Cell Biology 2023Quote: ... PARP14 MD1 was additionally purified by ion exchange chromatography using a HiTrap™ 5 mL SP HP (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
-
bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
-
bioRxiv - Biophysics 2023Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5 ml column (GE Healthcare, USA) for cation exchange ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
-
bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
-
bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was subjected to Ni-immobilized metal affinity chromatography (IMAC) (HisTrap FF Crude column, 5 ml, GE Healthcare). Proteins were eluted with a linear gradient of imidazole (10-500 mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... Sortase ligated PZA was purified in a single step over a 5-ml SP anion exchange column (GE Healthcare) to minimize protein degradation ...
-
bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... purified I53-50B.4PT1 was immobilized on Ni2+-NTA resin in a 5 mL HisTrap HP column (GE Healthcare) equilibrated with the following buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... Commercial human fibrinogen (America Diagnostica Inc) was desalted on a 5-ml HiTrap desalting column (GE Healthcare, IL, USA), eluted at a constant flow rate (1 ml/min ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant was supplemented with 10 mM imidazole and loaded onto a 5 ml Ni-NTA column (GE Healthcare). Beads were first washed with HBS containing 0.01% (w/v ...
-
bioRxiv - Bioengineering 2024Quote: ... A second protein purification step was performed using a cation-exchange column (HiTrap SP HP, 5 ml, GE Healthcare) with a high salt elution buffer (50 mM Tris–HCl at pH 7.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through 0.8 µm membranes and applied to a HisTrap HP 5 mL column (GE Healthcare) pre-equilibrated with Buffer A ...
-
bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
-
bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
-
bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
-
bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
-
bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
-
bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
-
bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
-
bioRxiv - Microbiology 2021Quote: ... The resultant eluate was filtered through a 0.45 μm filtration membrane and then loaded onto a 5 ml SOURCE 15S column (GE Healthcare) equilibrated with buffer I ...
-
bioRxiv - Biophysics 2020Quote: IgG was isolated from porcine sera by passing the sera over a MabSelect 5 mL HiTrap Column (GE Life Sciences), washing with two column volumes of tris-buffered saline and elution with 0.1 M glycine ...
-
bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
-
bioRxiv - Microbiology 2020Quote: ... The cleared membrane fraction was loaded with a flow of 0.8 mL/min on a pre-equilibrated 5 mL StrepTrap column (GE healthcare) mounted on as AktaPurifier system (GE healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: ... radioactive signal was impressed on a FUJIFILM Storage Phosphor screen for 5 hours and acquired with Typhon Trio (GE healthcare).
-
bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was loaded onto a 5-ml bed volume of HiTrap Heparin column (GE Healthcare, Pittsburgh, PA, USA) pre-equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... the sample was concentrated to 5 ml and loaded over the HiLoad 26/600 Superdex 200 prep grade (GE Healthcare). In order to clear the impurities which had been eluted with the complex ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied onto a 5 ml MBPtrap column (GE Healthcare). The column was washed with 10 CV of lysis buffer and the protein was eluted with 5 CV of lysis buffer supplemented with 15 mM maltose ...
-
bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
-
bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
-
bioRxiv - Developmental Biology 2020Quote: ... the supernatant was boiled at 98 °C for 5 min, applied to SDS page gel (SuperSep, WAKO) and transferred onto a 0.2 µM nitrocellulose blotting membrane (GE Healthcare). After incubation with blocking buffer (5% milk in tris-buffered saline with 0.1% Tween20 ...
-
bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
-
bioRxiv - Genomics 2020Quote: ... 20 µl PCR products were subsequently immobilized to 5 µl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and then were finally annealed to 1 µl sequencing primer (5 μM ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
-
bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
-
bioRxiv - Bioengineering 2021Quote: The yielded supernatant was passed through a 0.2 μm filter and loaded with a flow rate of 0.5 mL/min on a 5 mL HisTrap HP column (GE Healthcare) equilibrated with 12 CV binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... peak fractions were diluted to 100 mM NaCl and loaded onto a 5 ml HiTrap Q XL column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.3 ...
-
bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
-
bioRxiv - Biophysics 2021Quote: ... Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare) using a linear gradient from 150 mM NaCl to 1 M NaCl in buffer A ...
-
bioRxiv - Neuroscience 2020Quote: ... and 5 μL of Amersham ECL Rainbow Marker was loaded as a molecular weight standard (GE Life Sciences, Pittsburgh, PA). Proteins were transferred onto nitrocellulose and incubated with primary antibody ...