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Citations for GE Life Sciences :
3251 - 3300 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE Healthcare), which was then inucbated over-night with primary antibodies followed by a 1-hour incubation with fluorescent secondary antibodies (Table 1) ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins of interest were detected using Chemiluminescence by adding Amersham Enhanced chemiluminescence (ECL) detection reagent (GE healthcare). For quantification of Yops-secretion ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Molecular Biology 2024Quote: ... The recovered proteins after cleavage underwent size exclusion chromatography using a Superdex S75/300 column (GE Healthcare) in phosphate-based buffer [200 mM sodium phosphate pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Microbiology 2023Quote: Protein samples were separated on 10–15% polyacrylamide gels and transferred to Amersham nitrocellulose membranes (GE healthcare) by Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by SEC using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Biochemistry 2022Quote: ... For crystallography proteins were purified further via size-exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were pre-incubated with 60 uL of protein A-Agarose beads (GE Healthcare, Chicago, USA) + Salmon Sperm DNA (Invitrogen #15632-011 ...
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bioRxiv - Microbiology 2022Quote: Proteins were purified by immobilized metal affinity chromatography (IMAC) using Ni-Sepharose excel resin (GE Healthcare, USA). The column was first washed with 5 column volumes of loading buffer (50 mM tris(hydroxymethyl)aminomethane (TRIS) ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed proteins were concentrated and purified by SEC (HiLoad Superdex-200 16/600 column, GE Healthcare) into tau buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were concentrated and loaded onto a Superdex 200 column (10/300 GL; GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were immobilized by blotting them onto an Amersham Protran Premium nitrocellulose membrane (GE Healthcare Life Sciences) in cold 1 x Tris-Glycine ...
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bioRxiv - Plant Biology 2023Quote: ... and the eluted proteins were further purified via gel filtration chromatography with a superdex 200 (GE Healthcare) column ...
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bioRxiv - Microbiology 2023Quote: ... The pull-down of biotinylated proteins was performed using 100 µl StrAv Mag Sepharose™ (GE Healthcare) for each condition and incubated in a rotation wheel for 3h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was transferred to an Amersham™ Hybond™ P0.45 polyvinylidene difluoride (PVDF) membrane (GE Healthcare) in 1x Tris/Glycine transfer buffer (National diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were pooled and concentrated before separation on a Superdex 200 16/60 column (GE Healthcare) equilibrated in GF2 Buffer (50 mM Tris-HCl pH 7.5 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were concentrated and gel-filtrated on a SuperdexTM 75 size exclusion chromatography column (GE Healthcare) equilibrated with stock buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples with indicated concentrations were loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare, USA) pre-equilibrated with gel filtration buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS/PAGE gel and transferred to Protran nitrocellulose membrane (0.2 μm, GE Healthcare). Immunoblotting was performed by incubating with primary antibodies diluted in Tris-buffered saline (20mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL of protein was injected into the Superdex 75 or Superdex200 size exclusion column (GE Healthcare) (volume less than 900 µL for preparative runs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Acetylated LCas12a proteins were purified following the steps of Ni-NTA column (HisTap™ HP, GE Healthcare), ion exchange chromatography (HiTrap™ SP HP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
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bioRxiv - Biochemistry 2023Quote: Proteins were analyzed on a Superdex 200 10/300 GL column from GE healthcare (Chicago, IL, USA) equilibrated using a mobile phase containing 150 mM KCl and 50 mM Tris-HCl pH 7.6 run at 0.4 ml/min ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare) and stored in 20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was subsequently purified using a Superdex75 gel-filtration column (GE Healthcare, Chicago, IL, USA). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...