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Citations for GE Life Sciences :
3251 - 3300 of 4116 citations for Enterovirus CoxB3 VP1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were further purified via size exclusion chromatography using a GE Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... then separated by fast protein liquid chromatography system using HiTrap SP HP column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Biochemistry 2023Quote: ... The protein monomers were separated from oligomers by size exclusion chromatography on a Superdex 200 column (120 mL, GE Healthcare) equilibrated with buffer containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from extracts were separated on SDS polyacrylamide gels and blotted onto Nitrocellulose membrane (Hybond ECL Cat#RPN2020D, GE Healthcare). Membranes were blocked using Skimmed Milk 5% ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was then purified on a HiPrep™ 16/60 Sephacryl S-500 HR gel filtration column (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins (30 µg/lane) were resolved by SDS-PAGE and electro-transferred to the Hybond-P PVDF membranes (GE Healthcare). Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged protein was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare), eluted in buffer E (25 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... The protein sample was further purified by FPLC using a Superose® 6 Increase 10/300 GL column (GE Healthcare) equilibrated in sizing buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the protein of interest were applied to a Sephacryl 300 16/60 gel filtration column (GE Healthcare) and eluted with buffer D (10 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... and proteins were collected from culture supernatants and purified using the HisTrap HP column (GE Healthcare Life Sciences, Pittsburgh, PA) as described previously 33 ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... the labeled proteins were verified by running an SDS-PAGE and scanning using a Typhoon scanner (GE Healthcare, Danderyd, Sweden).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and proteins were transferred to Cytiva Amersham™ Protran™ 0.45 µm NC nitrocellulose blotting membranes (GE Healthcare, Munich, Germany). After blocking with 5% bovine serum albumin (Roth ...
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bioRxiv - Immunology 2024Quote: ... The complexes of SH-01AF594 with C3d and Fab 3E12 were obtained by concentrating ∼1 mg of either protein with 300µg of SH-01AF594 to ∼200µL before removing the excess protein via SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated and loaded onto a S75 16/600 size exclusion chromatography column (GE Healthcare, Chicago, USA). After chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... with the exception that the protein eluted from the Ni-NTA column was loaded on HiTrap Heparin column (GE Healthcare) equilibrated with HP1 buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...