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Citations for GE Life Sciences :
3201 - 3250 of 4116 citations for Borrelia burgdorferi sensu stricto B31 P35 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was applied onto superdex200 increase 10/300 GL column via fast protein liquid chromatography (AKTA Pure, GE Healthcare) with buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μl of pre-equilibrated protein L beads (Capto™ L resin, GE Healthcare Cat no. 17547802 Cat. no. 17547803) was added and reaction was incubated for 90 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were concentrated and further purified by size exclusion chromatography on a Superdex 75 column (GE Healthcare Life Sciences) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the crude lysate proteins were separated by Tricine-SDS PAGE (16%) and blotted onto a PVDF membrane (AmerhamTMHybondTM, 0.2 µM PVDF; GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Plant Biology 2022Quote: ... The culture was harvested and the recombinant protein purified using glutathione resin according to the manufacturer’s instructions (GE healthcare, 17075601).
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
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bioRxiv - Immunology 2022Quote: ... The purified protein buffer was exchanged to PBS containing 0.5 mM TCEP using a HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved on 6% SDS-PAGE gel and transferred to a nitrocellulose membrane (0.45 μm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Plant Biology 2022Quote: ... Fifty micrograms of proteins were separated by SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE Healthcare). The membrane was probed with rabbit polyclonal antibodies against maize calreticulin ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualised using Amersham ECL Western Blotting Detection Reagent and the ImageQuant LAS4000 luminometer (GE Healthcare Life Sciences). βIII-tubulin relative band intensities were quantified using ImageStudio software (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and the soluble proteins were isolated by centrifugation (80,000g, 45 min, 4°C) and purified by cation exchange chromatography (HiTrap SP, GE Healthcare) with a gradient of 0-1M NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was separated from free dye using gel filtration on a Superdex 75 pg 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Microbiology 2024Quote: ... Purified YraN proteins or variants were finally injected and separated on a Superdex 75 16/600 sizing column (GE healthcare) equilibrated with 50 mM Na-Phosphate pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction solution was sequentially precipitated using protein A sepharose beads and Glutathione Sepharose beads (#17-0756-01, GE Healthcare). The resulting supernatant was stored at −20 °C until it was analyzed for the surface plasmon resonance (SPR ...
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bioRxiv - Microbiology 2024Quote: ... Flow-through containing the untagged proteins was concentrated and loaded into a Superdex-200 10/300 GL column (GE Healthcare) pre-equilibrated with storage buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was further purified by size exclusion chromatography using a HiLoad 16/60 Superdex 200 column (GE Healthcare). Fractions containing the appropriate sized HA protein were concentrated ...
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bioRxiv - Immunology 2023Quote: ... Eluted protein was concentrated and loaded onto Superdex 200 increase 10/300 size exclusion column (GE Healthcare Life Sciences, Sweden). The peak fractions were collected and concentrated ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were isolated from cleared lysates by incubating with 70 µl of streptavidin beads (17-5113-01, GE Healthcare) at 4°C for 3h ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates containing protein were concentrated to <500µL and size-exclusion was performed at 4°C using an ÄKTA Pure Protein Purification System (GE healthcare) and Superose® 6 Increase 100/300 GL column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... The small-scale reconstitution reactions were pooled after dilution and concentrated using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The sample was subjected to size exclusion chromatography (SEC ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified and buffer exchanged by size exclusion chromatography using HiLoad 16/600 Superdex-G-200 (GE Healthcare) gel filtration column to storage buffer (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... Separation was done on 8% bis-acrylamide gels in Tris-Glycine-SDS buffer and proteins were transferred on Amersham Protran 0.2 µm NC membranes (GE Healthcare) for western blotting ...
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bioRxiv - Biophysics 2022Quote: ... The biotinylated protein was separated from excess biotin on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in 1x PBS buffer (10 mM Na2HPO4·7H2O ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Cell Biology 2023Quote: ... and further purified by affinity chromatography on an affinity column containing the above mentioned connexin36 segment protein coupled to CNBr-activated Sepharose (14 mg to 2.5 g Sepharose 4B; GE Healthcare). The specificity of the affinity purified antibodies was tested on tissue extracts and cryostat sections (12 µm thick ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein was further purified by size exclusion chromatography using a Superdex 200 Increase 100/300 GL column (GE Healthcare). The purified scFv16 were pooled ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated proteins were captured from lysates (500 µg per replicate) using 10 μg streptavidin sepharose (GE healthcare, Waukesha, Wisconsin, USA) for 8 h ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified using size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL, GE Healthcare). The purified protein was split into aliquots and stored at -80 °C prior to the measurements.
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bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
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bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size exclusion chromatography using a Superdex 200 Increase 100/300 GL column (GE Healthcare). The purified scFv16 were concentrated ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Immunology 2023Quote: ... The separated proteins were blotted onto a nitrocellulose membrane (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Freiburg, Germany), blocked for the unspecific binding with 5% non-fatty milk and incubated overnight at 4 °C with the respective primary antibody ...
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bioRxiv - Immunology 2023Quote: IgG was purified from plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was loaded into a HiTrapTm Q HP anion exchange column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and eluted with ∼ 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with buffer E and concentrated to 10 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 4°C at 10,000 g for 30 minutes and the supernatants were used to purify target proteins by Ni NTA agarose (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...