Labshake search
Citations for GE Life Sciences :
3151 - 3200 of 3237 citations for 6 Methylimidazo 1 2 a pyridine HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified by affinity chromatography with nickel affinity columns (HisTrap 1 ml; Cytiva) using the Äkta pure system (GE Healthcare UK Ltd.). Elution was carried out at a flow rate of 1 ml/min with all solutions maintained at 4 °C and a linear imidazole concentration gradient from 30 mM to 530 mM.
-
bioRxiv - Neuroscience 2021Quote: ... plasma samples were centrifuged and 1 μL of each sample was placed onto a clean piece of nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
-
bioRxiv - Cell Biology 2020Quote: ... and WAVE2-WCA (419-497) were amplified from plasmid DNA and inserted into BamHI/SalI sites of pGEX-6P-1 (GE Healthcare, Munich, Germany). Plasmids pGEX-6P-2-cortactin (Lai et al. ...
-
bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
-
bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
-
bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
-
bioRxiv - Microbiology 2022Quote: ... High performance liquid chromatography (HPLC) was conducted using a HiTrap Q XL 1 mL Sepharose column on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). Proteins were eluted with starting buffer A followed by a gradually increasing concentration of buffer B (20 mM Bis-Tris ...
-
bioRxiv - Plant Biology 2022Quote: ... Acetonenitrile was then added to 70 % and a 10:1 ratio of magnetic beads (Hydrophilic-Part no: 45152105050250, GE Healthcare plus Hydrophobic-Part no: 65152105050250, GE Healthcare, Australia) were added and mixed with the solution ...
-
bioRxiv - Cell Biology 2024Quote: ... This was injected into a size-exclusion column (column size 24 cm x 1 cm) containing Sepharose 4B (84 nm pore size) using an AKTA start system (GE Healthcare Life Science) and eluted with PBS ...
-
bioRxiv - Microbiology 2022Quote: ... brasiliensis by co-cultivation with yeasts at a ratio of 1:10 in sterile Hybond N filters (GE Healthcare Life Science, Pittsburgh, PA) in IM solid medium (induction medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
-
bioRxiv - Immunology 2024Quote: ... 1:3 blood dilutions were made with sterile Roswell Park Memorial Institute (RPMI) 1640 medium (HyClone; GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 100 unit/mL penicillin and 100 mg/mL streptomycin (Gibco Life Technologies ...
-
Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
-
bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... NRP was measured by first spotting 1 μl of each sample on the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA). Dry membrane was immersed in NRP working solution (10mg/ml KMnO4 in ddH2O) ...
-
bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
-
Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... This oligo was conjugated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1- carboxylate (SMCC linker through NH2-NHS ester reaction. The SMCC-linked oligo was purified through Nap column (GE Healthcare Life Sciences). The purified SMCC-linked oligo was conjugated with OVA through cysteine (thiol)-maleimide group coupling ...
-
bioRxiv - Microbiology 2023Quote: ... The 35 mer DNA oligonucleotide probes were radiolabelled with 32P-ATP at 37°C for 1 hour and then purified over a G50 size exclusion column (GE Healthcare Life Sciences) following the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
-
bioRxiv - Genomics 2023Quote: ... was used at 1:5000.) Blots were incubated with chemiluminescent detection reagent (Amersham ECL™ Prime Western Blotting Detection Reagents, GE Healthcare #RPN2236) and imaged on an Amersham Imager 600 (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
-
bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V; anti-mouse, GE Healthcare, NA931V) for 2 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
-
bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
-
bioRxiv - Biochemistry 2024Quote: ... H2AX (total) (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... NHR2 was then purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences) and analyzed using SDS-PAGE ...
-
bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Whole genome DNA was amplified using 1 μl polymerase enzyme from the illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Cat# 25-6600-32) and 9 μl of sample with the following conditions ...
-
bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
-
bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
-
bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
-
bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 mL fractions corresponding to ∼10 mL of the elution peak were pooled and applied to a 1 mL StrepTrap column (GE Healthcare, #28-9075-46) pre-equilibrated with Buffer N with 2 mM DTT ...
-
bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:200 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, USA; https://www.gelifesciences.com). Rubisco protein content was quantified from SDS-page gels using ALPHA-Ease FC software (Alpha Innotech ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
-
bioRxiv - Plant Biology 2020Quote: ... Fifty seeds per replicate were sown in Petri dishes containing three layers of filter paper (Whatman grade 1, GE healthcare, Little Chalfont, United Kingdom) and imbibed with 4 mL of ultrapure water (UPW) ...
-
bioRxiv - Neuroscience 2020Quote: ... installed prone on the MRI bed and anaesthesia was immediately switched to 1% ISO in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified using a NiNTA purification system (HisTrap™ excel 1 mL) and size-exclusion chromatography using HiPrep™ 26/60 Sephacryl™ S-100 HR (GE Healthcare) in 10 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: A paper punch was used to make 3 mm diameter circles of Whatman™ Grade 1 180 μm thick cellulose filter paper (GE Healthcare Life Sciences), which were stored in sterile disposable petri dishes ...
-
bioRxiv - Cell Biology 2019Quote: ... Selected plakoglobin-containing fractions (Fig. 1d, fractions #8-11) were combined and loaded onto a 1 ml Resource Q anion exchange column (GE Healthcare Life Sciences, UK), equilibrated with 20 mM Tris pH 7.2 and 500 mM NaCl ...
-
bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Cell Biology 2019Quote: ... and Gabarapl2 (EcoRI/SalI) were amplified from cDNA derived from B16F10 melanoma cells and cloned in the vector pGEX-4T-1 (GE Healthcare, Chicago, Illinois, USA) into the sites indicated in parentheses ...
-
bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...
-
bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
-
bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...