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Citations for GE Life Sciences :
2951 - 3000 of 4264 citations for Recombinant Human PTHLH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The cleaved protein was then concentrated to 2.0 ml final concentration and loaded on the S200 column (GE healthcare). S200 size exclusion chromatography has yielded a single peak around 230 KDa ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The ion-exchange chromatography was performed for further protein purification with an anion-exchange column (Source 15Q, GE Healthcare). Then peak fractions were collected and stored at -80 °C before using.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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bioRxiv - Immunology 2019Quote: ... after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane, GE Healthcare Life Sciences) or PVDF membranes (Amersham Hybond P 0.45 µm PVDF Membrane ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified on an ion exchange column MonoS and gel filtration column Superose 6 (GE Healthcare). Purified protein was concentrated to ∼20 mg/ml in BRB20 buffer (20mM PIPES ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2019Quote: ... Protein at 50 µM was injected onto a Superdex 200 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg of total protein were run on a SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE Healthcare). After blocking in 5% (w/v ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysate was pre-incubated with 200 μL of rehydrated Protein A Sepharose CL-4B beads (GE Healthcare, Life Sciences) for 15 minutes at 4°C with rocking ...
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bioRxiv - Cell Biology 2019Quote: ... LEM2 proteins were further purified by size exclusion chromatography using the Superdex 75 16/60 column (GE Life Sciences) in storage buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... the desalted protein-containing fractions were pooled and loaded on a Q sepharose FF column XK16/32 (GE Healthcare). The protein-containing fractions in the flow-through of the column were again pooled ...
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bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: For Western blotting protein samples were resolved by 10% SDS-PAGE and transferred to a PVDF membrane (GE Healthcare) by semi-dry blotting ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The respective IgG2a antibodies were incubated with the PepSpot membrane at concentrations ranging from 0.5 µg/ml to 2µg/ml and detected by protein A-HRP (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of the proteins was performed by SDS-PAGE and size exclusion chromatography (Superdex200 10/300GL, GE Healthcare). The full amino acid sequences are presented in Supplementary Figure 2 ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... Samples were separated on 10–12% SDS-PAGE gels and proteins were transferred to Protran nitrocellulose membranes (GE Healthcare), after which membranes were stained with 0.3% w/v Ponceau S in 3% v/v acetic acid and digitized ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose Amersham Protran membrane (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and further purified using size exclusion chromatography (SEC) on an AKTA pure 25L protein purification system (GE Healthcare, USA) in buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Further purification of the proteins was achieved using a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) in 50 mM HEPES/NaOH pH7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by size exclusion chromatography using an S75 or S200 16/60 column (GE Healthcare) equilibrated in running buffer (10 mM Tris/HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was applied to size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE healthcare) in gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). nsp7 and nsp8 were purified in SEC-150 buffer ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 column (10/300 GL, GE Healthcare) equilibrated with 200 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified in one step by IMAC followed by size exclusion chromatography using ÅKTAxpress system (GE Healthcare) as previously described with some modifications (28) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... This mixture was then added to 30 μl of pre-equilibrated Protein G sepharose beads (GE Healthcare Life Science) and incubated ON under continuous agitation ...
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then further purified by size-exclusion chromatography on a 16/600 Superdex 200 column (GE Healthcare) equilibrated in either 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 HiLoad 16/60 (GE Healthcare) for size exclusion chromatography and elution was performed with 25mM Phosphate buffer pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were separated from unreacted fluorophores by passing over a Superpose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Plant Biology 2020Quote: ... The secreted protein was purified from the supernatant by Ni2+ affinity chromatography on a HisTrap Excel column (GE healthcare), equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).