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Citations for GE Life Sciences :
251 - 300 of 806 citations for Recombinant Human Metadherin GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The GST-ScLas1 mutants were further purified on a Heparin HP column (GE Healthcare) and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Biochemistry 2023Quote: ... The GST-tag was removed by an on-column cleavage using thrombin (GE Healthcare) at a final concentration of 1 unit/mL ...
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bioRxiv - Microbiology 2020Quote: His-tagged HexaPro was immobilized to a NiNTA sensorchip (GE Healthcare) to a level of ∼500 response units (RUs ...
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bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Biophysics 2021Quote: ... GST eluates were further purified with an anion exchange column (HiTrap Q HP, GE Healthcare) and the peak fractions were pooled for co-immunoprecipitation experiments ...
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bioRxiv - Plant Biology 2020Quote: ... The bound HY5 was detected by anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences). Membranes were developed and visualized as described above ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V(370-709) was purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom) and eluted with reduced glutathione (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved GST was separated by anion exchange chromatography (HiTrap Q HP 5 mL (GE Healthcare)) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by GST column purification using the ÄKTA start chromatography system (GE Healthcare, Chicago, IL). Cell lysate supernatant was added to the column containing nickel resin and it was recirculated across the column by a peristaltic pump overnight at 0.5 ml/min ...
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bioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified from Escherichia coli by glutathione-Sepharose 4B beads (GE Healthcare) and then washed with high salt buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion V5-Ydj1 was subjected to site-specific cleavage with PreScission Protease (GE Healthcare) to remove the GST tag ...
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bioRxiv - Cell Biology 2020Quote: ... while Dcp1a was eluted by excision of the GST moiety trough PreScission protease (GE Healthcare) incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion protein and His fusion protein were purified with glutathione-Sepharose 4B (GE Healthcare) and Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... GST-PDXP was batch-purified on a glutathione sepharose 4B resin (GE Healthcare, Uppsala, Sweden). After extensive washing with 25 column volumes of wash buffer (50 mM TEA ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 and affinity-purified with GST beads (#17-0756-01, GE Healthcare Life Sciences). 293T cells overexpressing indicated proteins were treated with DNA damage reagents and lysed ...
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bioRxiv - Biochemistry 2023Quote: ... The GST-ICD tryptophan mutations (“black” W22X) were purified through Glutathione Sepharose beads (GE Healthcare), followed by dialysis into pull-down buffer containing 100 mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... these proteins were mixed with GST-LAZY4ΔCCL and incubated with Glutathione Sepharose 4B (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... followed by pull-down of GST-Slp1(456-488aa) by glutathione Sepharose 4B (GE Healthcare). Purified GST-Slp1(456-488aa ...
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Bioengineering 2021Quote: ... PEGylated mEGFP tagged ferritin was purified with PD10 desalting columns (GE Healthcare) equilibrated with 20mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
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bioRxiv - Microbiology 2020Quote: ... HisMBP-tagged PurR was loaded onto a HisTrap FF column (GE Healthcare) equilibrated with Lysis Buffer on an AktaPure FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Biochemistry 2019Quote: ... The synthesized ltgA deletion gene was cloned into a GST-fusion pGEX-4T1 (GE Life Sciences) plasmid as described above ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by ultracentrifugation and supernatant was incubated with GST-4B beads (GE Life Sciences) for 1H ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... GST-SENP was captured and removed from the eluate by incubation with GSH beads (GE Healthcare). Purified PLpro was further concentrated using an Amicon 5 kDa spin filter to a final concentration of 1 mg/ml and stored at −80 °C (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: TFEB fragments were cloned in frame with GST in pGEX-2T (GE Healthcare, 27-1542-01). Overnight bacterial culture (E ...
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bioRxiv - Molecular Biology 2021Quote: ... the cleared supernatant was collected and incubated with 100 μl of GST Sepharose beads (GE Healthcare) for 1-2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST-GIP was purified by passage onto a glutathione-sepharose GSTrap HP 1ml column (GE Healthcare) with 50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare) were incubated with 1 ml of HeLa cell extract for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-A3C fusion enzyme containing slurry was treated with 0.02 units/μl thrombin (GE Healthcare) for a minimum of 4 hours at room temperature to release A3C from the GST tag ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was released from the GST beads by thrombin cleavage and purified by MonoQ (GE Healthcare) ion-exchange chromatography ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then applied to a 10 mL packed GST resin (GE Healthcare, Waukesha, WI) equilibrated in Wash Buffer [PBS with 250 mM NaCl and 1 mM DTT] ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) and purified with GST-BIN1_SH3 bound to Glutathione-Sepharose 4B beads (GE Healthcare). Human SH3 of BIN1 with GST tag (GST-SH3 ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...