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Citations for GE Life Sciences :
251 - 300 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg, GE healthcare) pre-equilibrated with the buffer containing 20mM HEPES pH 7.5 and 150 mM NaCl (Supplementary Fig ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant proteins were purified by Ni2+-affinity chromatography using Histrap HP FPLC columns (GE Healthcare) and a size-exclusion chromatography using a HiLoad 16/60 200 PG column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Recombinant IgG mAbs were purified using HiTrap Protein A columns (Cytiva/GE Healthcare Life Sciences, 17040303).
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were purified from the filtrated supernatants using a HisTrap excel column (GE Healthcare). The purified proteins were biotinylated in vitro using the BirA enzyme in the presence of 0.5 mM Biotin and 10 mM ATP ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant proteins were then eluted from a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-Trap Q (GE Healthcare), and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Microbiology 2021Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...