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Citations for GE Life Sciences :
251 - 300 of 442 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... RNA was transferred onto Hybond N+ positive charged nylon membranes (Cat# RPN303B; GE Healthcare, Little Chalfont, UK) using semi-dry transfer and cross-linked using ultraviolet light ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... Gels were rinsed in 0.5x TBE and RNA was transferred onto a Hybond N+ membrane (GE Healthcare) in 0.5x TBE by semi-dry transfer (Bio-Rad Transblot ...
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bioRxiv - Genetics 2022Quote: ... The RNA was transferred overnight by capillarity to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) and covalently bound to the membrane using a Stratalinker UV crosslinker ...
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bioRxiv - Genetics 2023Quote: ... The DNA fragments were UV-nicked prior to transfer to Hybond N + Nylon membrane (GE Healthcare, RPN203B) as described in the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... separated on 1.2 or 1.8% formaldehyde-MOPS agarose gels prior transfer onto Hybond-N+ membranes (GE Healthcare) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... consisting of a Protran nitrocellulose membrane (Whatman) followed by a Hybond N+ nylon membrane (GE Healthcare Bioscience). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... The RNA was transferred overnight by capillarity to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) and covalently bound to the membrane using a Stratalinker UV crosslinker ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was resolved through denaturing polyacrylamide/urea gels and transferred to Hybond N+ membranes (GE Healthcare, UK) using standard protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of total RNA was dropped onto an Amersham Hybond-N+ membrane (GE Healthcare, cat# RPN203B), then the membrane was briefly air dried and crosslinked using Stratalinker 2400 UV Crosslinker twice using Autocrosslink mode ...
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bioRxiv - Biochemistry 2021Quote: Fractions containing the dIG8-CC protein were then buffer-exchanged to buffer B (20mM Tris·HCl, 150 mM sodium chloride, pH 7.5) in a HiPrep 26/10 desalting column (GE Healthcare), and incubated overnight at 4°C with inhouse-produced His6-tagged TEV peptidase at a peptidase:substrate ratio of 1:20 (w/w ...
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bioRxiv - Immunology 2020Quote: ... The trimer fraction was purified by size exclusion chromatography on a Superdex 200 10/300 GL column in TBS + 0.1% DDM + 0.003% sodium deoxycholate using an ÄKTA Pure 25L HPLC instrument (GE Healthcare) at a flow rate of 0.75 ml/min.
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bioRxiv - Molecular Biology 2021Quote: ... The reaction products were dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) followed by hybridized at 50 °C with end-labeled (CCCTAA)4 to detect C-circles or (TTAGGG)4 to detect G-circles.
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bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) and hybridized with a telomere (or a tubulin ...
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bioRxiv - Immunology 2021Quote: ... each ectodomain variant was chemically cross-linked to N-hydroxysuccinimide (NHS)-activated Sepharose beads (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and UNC- 16(1-506) was covalently coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: RNA was separated on a 16% polyacrylamide gel and transferred to the Amersham Hybond-N+ membrane (GE Healthcare). Probes tRNA-Glu-TTC ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA gel was electroblotted onto an Amersham Hybond-N neutral membrane (GE Healthcare UK Limited, Buckinghamshire, UK) at 180 mA for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
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bioRxiv - Immunology 2021Quote: ... mRNA was mixed with RNA loading buffer and then carefully spotted onto a Hybond-N+ membrane (GE Healthcare), followed by UV crosslinking ...
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bioRxiv - Microbiology 2022Quote: ... Separated DNA fragments were transferred to the nitrocellulose membranes (Amersham Hybond-N+; GE Healthcare UK Ltd., Buckinghamshire, UK) by capillary transfer ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved on a 0.8% agarose gel and transferred onto a Hybond-N positively charged membrane (GE Healthcare, UK). Oligonucleotide primers were used to amplify a Maxi-probe (SMD359/6 ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Cell Biology 2023Quote: ... as a model of pre-adipocytes [30] were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (Nacalai ...
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bioRxiv - Cell Biology 2024Quote: ... Public Health England, London, UK) were maintained in DMEM (Nacalai, Osaka, Japan) with 10% fetal bovine serum (FBS) (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (P/S ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Cell Biology 2024Quote: Samples were separated electrophoretically on a 12.5% sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) and transferred to Polyvinylidene Difluoride (PVDF, GE Healthcare) membranes at 60 mA per membrane for 90 min in a semi-dry transfer unit (TE77X Hoefer) ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Microbiology 2024Quote: ... The deacylated tRNA was ethanol precipitated and dissolved in monoQ buffer A (40 mM sodium phosphate buffer, pH 7.0) and separated on the 8-ml MonoQ column (10/100, GE Healthcare), using two-step linear gradient of buffer B (A with 1M NaCl) ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of lyophilized Aβ was resuspended in 700 µl GE buffer pH 8.5 (6 M guanidinium hydrochloride, 20 mM sodium phosphate) and loaded onto a Superdex 75 SEC column (GE Healthcare) equilibrated with aggregation buffer (20mM sodium phosphate ...
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bioRxiv - Neuroscience 2022Quote: ... The Biorad Bio-Dot apparatus was assembled according to the manufacturer’s protocol with Hybond N+ nylon membrane (GE Healthcare) on the top ...
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bioRxiv - Biochemistry 2022Quote: RNA samples separated by 11% WIDE Range Gel SDS-PAGE were transferred onto a Hybond-N+ membrane (GE Healthcare) and hybridized with biotinylated oligonucleotide(s ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...