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Citations for GE Life Sciences :
251 - 300 of 536 citations for Galectin 9 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-A3C fusion enzyme containing slurry was treated with 0.02 units/μl thrombin (GE Healthcare) for a minimum of 4 hours at room temperature to release A3C from the GST tag ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Biochemistry 2022Quote: Purified GST-tagged DIDATRX and DIDATRX fragments were immobilized on Glutathione sepharose 4B beads (GE Healthcare) under saturating conditions in binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was released from the GST beads by thrombin cleavage and purified by MonoQ (GE Healthcare) ion-exchange chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then applied to a 10 mL packed GST resin (GE Healthcare, Waukesha, WI) equilibrated in Wash Buffer [PBS with 250 mM NaCl and 1 mM DTT] ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) and purified with GST-BIN1_SH3 bound to Glutathione-Sepharose 4B beads (GE Healthcare). Human SH3 of BIN1 with GST tag (GST-SH3 ...
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bioRxiv - Neuroscience 2020Quote: ... HepG2 and HEK293 cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 μg of GST-FG-repeat were incubated with 50 μL Glutathione Sepharose 4B beads (GE healthcare) at 4°C for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... GST pulldowns were done as described in (Bertran et al., 2011) using glutathione agarose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... to remove the GST tag and further purified by gel filtration (Superose 6, 10/30; GE Healthcare). For cryo-EM investigation ...
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bioRxiv - Synthetic Biology 2021Quote: ... a glutathione-S-transferase (GST) (64) and thrombin sequence derived from pGEX-2T (GE Healthcare, Chicago, IL), and a maltose binding protein (MBP ...
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bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Cell Biology 2019Quote: ... or GST-Bud61-364 were incubated with HBSEP-equilibrated glutathione-coupled sepharose beads (GE Healthcare, Freiburg, Germany), for 30 min under rotation in the cold ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... GST beads - (Glutathione Sepharose 4b beads, particle size 45 µm - 165 µm, GE Healthcare 17-0756-05) were incubated with the lysate overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were fused in-frame behind GST on a pGex2T vector (GE Healthcare, Buckinghamshire, UK). 6xHis-tagged fragments were amplified from yeast genomic DNA using primers containing SfiI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Microbiology 2023Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare). Quantification of the EtBr and radioactive signals was performed with Fiji software (80) ...
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bioRxiv - Plant Biology 2019Quote: ... Glutathione S transferase (GST)-tagged recombinant proteins were purified using glutathione sepharose 4B (GE Healthcare, #17-0756-01) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare; catalog no. 17-0756), according to the batch purification method described in the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-fused IQGAP1-N and IQGAP1-C were purified using Glutathione Sepharose 4B (17-0756-01; GE Healthcare). Next ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... GST-tagged and His-tagged proteins were affinity-purified using glutathione agarose beads (GE Healthcare, Pittsburgh, PA, USA) and Ni-NTA affinity agarose beads (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... Scd1 (aa 10-872) and Rga4 (aa 389-1150) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare), expressed in BL21 (DE3 or Rosetta ...
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bioRxiv - Cell Biology 2020Quote: ... western blot based GST pull-down assay was performed in which Glutathione Sepharose® 4B beads (GE Healthcare) was coupled with 10 µg of purified r14-3-3I protein in buffer A (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...