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Citations for GE Life Sciences :
251 - 300 of 4684 citations for Angiotensin II receptor AT 2R Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... RBD was purified from the supernatants by nickel affinity chromatography with HisTrap Excel columns (GE Healthcare Life Sciences). For protein production used in crystallization trials ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The vector was purified from the clarified lysate by affinity chromatography using a HiTrap heparin column (GE Healthcare), ultracentrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2023Quote: ... His6-HsCHIP21-154-TPR was purified by Ni2+-affinity chromatography using a 5mL HisTrap HP column (GE Healthcare) loaded in 25 mM HEPES pH 7.5 and 50 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Immunology 2024Quote: ... and fermentation supernatants were purified by Protein-A affinity chromatography using MabSelect™ column (GE Healthcare, Princeton, NJ). The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-rabbit antibody (sheep polyclonal, used at 1:3000, GE Healthcare) and HRP-conjugated anti-chicken antibody (donkey polyclonal ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated anti-rabbit IgG donkey polyclonal antibody (GE Healthcare, Chicago, IL; #NA934V), HRP-conjugated anti-sheep IgG donkey polyclonal antibody (Chemicon International ...
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bioRxiv - Microbiology 2021Quote: ... polyclonal SagA serum and HRP conjugated anti-Rabbit IgG (GE Healthcare, NA 934V) served as primary and secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... All GST-B3 fusion proteins were expressed in E.coli BL21(DE3) cells and were purified by gluthathione-Sepharose affinity chromatography according to the manufacturer’s recommendations (GE Healthcare). The proteins were eluted in elution buffer (20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... the GST-PACRGΔ1-69:MEIG1 complex was purified by affinity chromatography using 1.5 mL of glutathione-sepharose resin (GE Healthcare) washed in Buffer A (50 mM of HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using Ni-NTA affinity resin (Quiagen) and followed with SEC (Superose 6 Increase, GE Life Sciences), as previously described10 ...
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bioRxiv - Plant Biology 2020Quote: ... The secreted protein was purified from the supernatant by Ni2+ affinity chromatography on a HisTrap Excel column (GE healthcare), equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... soluble proteins were purified by affinity chromatography using a HisTrap Ni-NTA column (ÄKTA pure FPLC system, GE Healthcare) equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Protein was purified by Ni2+ affinity chromatography followed by size-exclusion chromatography on a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was purified from the clarified supernatant using a Ni-NTA affinity chromatography column (HisTrap FF GE Healthcare) with binding buffer and eluted through on column cleavage with HRV 3C protease ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Synthetic Biology 2021Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A (20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Secreted Fabs were purified from cell supernatants harvested four days post-transfection using Ni2+-NTA affinity chromatography (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Immunology 2021Quote: ... IgG from the hybridoma cell line supernatants was purified by affinity chromatography using protein G columns (GE Life Sciences). Purified H5.28 and H5.31 IgG generated from hybridomas was used for EC50 binding measurements ...
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bioRxiv - Genetics 2022Quote: ... Mutant and wild-type DASH complexes were first purified via affinity chromatography using either FLAG resin (GE Healthcare Bioscience) or Nickel resin (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A at a constant flow rate of 1 mL/min ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated proteins were purified based on nickel affinity purification by AKTA Prime Plus automated purification system (GE Healthcare). The sample passed over a 1 mL Nickel-Sepharose (HisTrap HP ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... GST-FlgN and GST-FliJ were purified by GST affinity chromatography with a glutathione Sepharose 4B column (GE Healthcare) from the soluble fractions from the Salmonella SJW1368 strain transformed with a pGEX-6p-1-based plasmid as described previously29 ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions with >95% pure protein were pooled and re-purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of 0 – 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... XMAP215-SNAP-TEV-7xHis-StrepII or XMAP215-TEV-GFP-7xHis-StrepII was first affinity purified with StrepTrap HP (GE Healthcare) with binding buffer (50mM NaPO4 ...
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bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...