Labshake search
Citations for GE Life Sciences :
251 - 300 of 503 citations for 7 Oxa 12 thia 3 aza spiro 5.6 dodecane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
-
bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
-
bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
-
bioRxiv - Cell Biology 2020Quote: ... infected cells were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix as described ...
-
Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligandsbioRxiv - Immunology 2021Quote: ... the pellet was dissolved in 3 ml of 30% Percoll (Percoll PLUS, GE Healthcare) and carefully layered on top of 70% Percoll and immediately centrifuged (650 rcf for 20 min) ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
-
bioRxiv - Biophysics 2021Quote: ... followed by concentrating the sample (VivaSpin 6 MWCO 3 kDa cut-off, GE Healthcare) to 8 μM ...
-
bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
-
bioRxiv - Biochemistry 2020Quote: ... A volume of 3 mL of Glutathione Sepharose 4B resin (17075605, GE Life Sciences) was added to the soluble fraction and incubated for a minimum of 6 hours at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... chromatin lysates were incubated for 3 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
-
bioRxiv - Cell Biology 2022Quote: ... were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare) or Alexa FluorTM (AF ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on customized microarray chip using commercially available hybridization platform (Agilent ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) which had been equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 2–3 minutes with ECL detection reagent (Amersham GE Healthcare), and were then developed using a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
-
bioRxiv - Biochemistry 2024Quote: ... GST-tagged constructs were cloned into a modified form of pGEX4T-3 (GE Healthcare) with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV ...
-
bioRxiv - Molecular Biology 2019Quote: ... cell lysates corresponding to 2×105 cells in the case of in vivo experiments or 4×105 per sample in the case of nuclease-treated lysates were analyzed by electrophoresis on 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science). The proteins of interest were probed with antibodies against the following proteins ...
-
bioRxiv - Neuroscience 2021Quote: The SH-SY5Y cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and maintained in MEM/F-12 mixture containing 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
-
bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
-
bioRxiv - Neuroscience 2020Quote: The human neuroblastoma SH-SY5Y cells purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) were maintained in MEM/F-12 mixture containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
-
Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10–12 % SDS-polyacrylamide gel electrophoresis gel (SDS-PAGE; Carl Roth) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Membranes were blocked for 1 h with 5 % non-fat milk in PBS-T (PBS with 0.1 % Tween-20 ...
-
bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
-
bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
-
bioRxiv - Cell Biology 2022Quote: ... For immunoblotting the platelet lysates were separated by a SDS-polyacrylamide gel electrophoresis (12 % SDS-polyacrylamide gel) and electro-transferred onto a nitrocellulose blotting membrane (GE Healthcare Life sciences). After blotting ...
-
bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2021Quote: ... The cDNA was labelled individually with both Cy-3 and Cy-5 dyes (GE Healthcare) using Superscript III reverse transcriptase (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was incubated with 3 mL equilibrated Glutathione-Sepharose 4B beads (GE Healthcare) for 45 min at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: Whole brain images were acquired with the 3 T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin-Madison ...
-
bioRxiv - Biochemistry 2022Quote: ... and 3 mM DTT and was loaded onto a MonoS 10/100 column (GE Healthcare). The protein was eluted with an increasing salt (KCl ...
-
bioRxiv - Neuroscience 2022Quote: Images were acquired using a 3 tesla MRI scanner (Discovery MR750; GE Healthcare, Waukesha, Wisconsin) equipped with a 32-channel head coil ...
-
bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The amplification product of WRKY45 was cloned into pGEX-6P-3 vector (GE Healthcare, USA) that was cleaved with BamHI ...
-
bioRxiv - Neuroscience 2023Quote: Whole brain images were acquired with a 3-T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison ...
-
bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
-
bioRxiv - Plant Biology 2024Quote: ... for 3 h and then scanned by Typhoon FLA 9000 (GE Healthcare co., Chicago, USA). 32P uptake rate was calculated based on a calibration curve for 32P ...
-
bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
-
bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
-
bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
-
bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
-
bioRxiv - Biophysics 2021Quote: ... The supernatant was then incubated with 3 ml pre-equilibrated Ni-NTA resin (GE Healthcare, USA).
-
bioRxiv - Microbiology 2019Quote: ... First dimension isoelectric focusing (IEF) run was performed using Ettan IPGphor 3 IEF System (GE Healthcare) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Supplementary Table 2 for a list of antibodies used in this study ...
-
bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Extended Data Table 1 for a list of antibodies used in this study ...
-
bioRxiv - Biophysics 2020Quote: ... the sample was loaded onto a freshly poured 3 mL G50 Sephadex column (GE Life Sciences) equilibrated in CoWB ...
-
bioRxiv - Microbiology 2019Quote: ... Three additional siRNAs targeting the 3’-UTR of KSRP mRNA (siKSRPe) were synthesized (Dharmacon, GE Healthcare) and pooled to a 20 µM stock solution ...