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Citations for GE Life Sciences :
251 - 300 of 4190 citations for 6 METHYL 7 1 METHYL 2 OXO PROPOXY 2 3 DIHYDRO 1H CYCLOPENTA C CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1-2 minutes in darkness then exposed and imaged using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... for 1-7 days before acquiring radiograms using Typhoon 5 Imager (GE Healthcare). Densitometry was carried out using ImageJ53 ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and loaded on a HisTrap column (GE Healthcare, Chicago, Illinois, United States) for purification ...
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bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
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bioRxiv - Biochemistry 2019Quote: Size-exclusion chromatography was performed at 4 °C on an AKTA FPLC (GE Healthcare). Approximately 10 mg of apoferritin (Sigma A3660-1VL ...
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bioRxiv - Neuroscience 2023Quote: ... with MOPS buffer and transferred overnight at 4°C onto nitrocellulose membranes (GE Healthcare) with Tris-Glycine transfer buffer (25 mM Tris-base ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM β-mercaptoethanol) on an ÅKTA system (GE Healthcare). PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM TCEP) using a HiPrep Desalting column (GE Healthcare) and stored over night at 4°C in final buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 µl 0.5 mM NiCl2 ...
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bioRxiv - Genomics 2022Quote: ... HRP-linked F(ab’)2 fragment (NA9340) from GE Healthcare/Amersham.
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bioRxiv - Systems Biology 2023Quote: ... for 2 min and detected on an ImageQuant (GE Healthcare). The proteins were quantified using ImageQuant TL software (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Radiographic contrast agent (Omnipaque, 2% diluted in saline, GE Healthcare) was used in conjunction with an Elite CFD radiographic C-arm (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were imaged using API Deltavision 2 (Applied Precision Instruments) widefield microscope using YFP filter set at 100x magnification.
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bioRxiv - Microbiology 2023Quote: ... HRP-linked F (ab’)2 Fragment (GE Healthcare Bio-Sciences) and 1-Step™ TMB ELISA Substrate Solutions (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 peak eluent was collected and loaded on 2 × 1 mL Heparin-Sepharose columns (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Microbiology 2020Quote: ... 7 cm strips (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma#255599) ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...