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Citations for GE Life Sciences :
251 - 300 of 3892 citations for 6 4 Methoxyphenyl 2 oxo 1 2 dihydro 3 pyridinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl2) using the PD-10 desalting columns (GE Healthcare, Piscataway, NJ). Quantification of VP2 particles was determined by absorbance at A280 with NanoDrop (NanoDrop2000 ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Biochemistry 2022Quote: MCherry-kindlin 2 was bound on a HisTrap FF crude column (GE Healthcare) and eluted with 300 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2022Quote: ... MBNL1 (amino acids 1-269 of MBNL140) was obtained in pGEX-6P1 (GE Healthcare). Plasmids were transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... or incubated with 1 ml Ni2+ slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) per 1 L expression medium for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Sepharose™ 6 10/300 GL resin (30 cm x 1 cm; GE Healthcare) was equilibrated in PBS buffer (10 mM orthophosphate ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 mM NaCl and 2 mM DTT on a Superdex 200 column (GE Healthcare). In case of individual purification of PHF14 and HMG20A ...
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bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Microbiology 2019Quote: ... tuberculosis CFP was purified and concentrated using 2-D Clean-Up Kit (GE Healthcare) and resuspended in 125 µl of rehydration solution (urea 7M ...
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bioRxiv - Biochemistry 2020Quote: ... Supernates were loaded by gravity onto a Ni-2+-charged column (HisTrapHP, GE Healthcare) equilibrated with Buffer E ...
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bioRxiv - Microbiology 2020Quote: ... A 20 μL sample of Precission protease (2 U/μL; catalogue # 27084301, GE Healthcare) was added and beads were incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM 1,4-dithiothreitol) using Illustra NAP columns (GE Healthcare). Apo AfAgo sample was measured in the same buffer containing 500 mM NaCl.
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bioRxiv - Microbiology 2020Quote: ... SARS2-CoV-2 RBD-His was immobilized to a CM5 sensor chip (GE Healthcare). Serial dilutions of purified recombinant Nanosota-1 drugs were injected at different concentrations ...
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bioRxiv - Plant Biology 2021Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... spots were analyzed using Image Master 2-D Platinum image analysis software (GE Healthcare). One-way factor ANOVA (P < 0.05 ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of each reaction was spotted onto Protran BA85 nitrocellulose (GE Healthcare) via pipette or a replicator pinning tool (V&P Scientific ...
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mM EDTA) and electroblotted onto a nylon (N+) membrane (GE Healthcare RPN203B) at 20 V for 90 min at 4 °C in 0.5x TBE buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 2 mL Ni-Sepharose High Performance slurry (GE Healthcare) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare) for 4 h ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μg of patient-derived AABs were dotted on a nitrocellulose membrane (GE Healthcare), membranes were probed with mouse anti-human IgG1-POD ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
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bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined using commercially available 2-D quant kit (GE Healthcare Life Sciences). Sample quality was confirmed using NuPAGE® protein gels stained with fluorescent SyproRuby protein stain (Thermo Fisher).
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bioRxiv - Genomics 2019Quote: ... The flow-through was then loaded onto a 2 mL HiTrap SP column (GE Healthcare) in 17% IEX-B buffer ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...