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Citations for GE Life Sciences :
251 - 300 of 3676 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Molecular Biology 2020Quote: ... and subcloned into the pGEX-6P-3 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Biophysics 2024Quote: ... we labeled tubulin with Cy-3 (PA23001, GE Healthcare) for the optical trap experiments or ATTO647N (AD 647N ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with Ni-nitrilotriacetic acid (NTA) affinity chromatography (HisTrap HP columns from GE Healthcare) in an AKTA purifier (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Overnight trichloroacetic acid/acetone precipitation was performed with 2D Clean-Up kits (GE Healthcare) as instructed by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... The protein was first purified with a Ni2+-nitrilotriacetic acid (NTA) resin (GE Healthcare,) using an elution buffer consisting of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... a modified trichloroacetic acid–acetone precipitation method (Clean-Up Kit; GE Healthcare, Munich, Germany) was used ...
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bioRxiv - Microbiology 2023Quote: ... Radioactively labelled proteins or nucleic acids were visualised using storage phosphor screens (GE Healthcare) and a Typhoon 9400 imager (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... GSTrap columns (5 mL; GE Healthcare) were used with an Akta Prime FPLC system (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of HisTrap (GE Healthcare) was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare) ...
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bioRxiv - Physiology 2022Quote: ... including 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 peak eluent was collected and loaded on 2 × 1 mL Heparin-Sepharose columns (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... the H3 and H4 monomers were purified using a mono-Q HR 5/5 column (GE healthcare) and SP ion exchange resin (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... All the nucleosomal DNA fragments were purified using ion-exchange chromatography (Source Q 5/5, GE Healthcare) and isopropanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in Nap1 chromatography buffer (20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The reduced 5′ thiol-modified ssDNA was purified into water using NAP-5 columns (GE Life Sciences). The reduced 5’ thiol-modified ssDNA was spin vacuum dried ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...