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Citations for GE Life Sciences :
251 - 300 of 2539 citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...
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bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare): Hybridization was performed overnight in the same buffer containing a 32P-labeled probe ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and BSA was replaced by 2% charcoal dextran-treated FBS (HyClone, GE Healthcare Life Sciences).
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bioRxiv - Immunology 2022Quote: ... was synthesized by ABclonal Technology and got the 2 mg/mL purified antigen through Ni-IDA (GE Healthcare, USA). The mouse polyclonal antibodies against two parts of PGRP-S2 (NM_001163716.1) ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We purified all samples using a 2-D Clean-Up Kit (GE Healthcare, Uppsala, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... lactose and 2’FL to RhLNBBP was measured using a Microcal ITC200 calorimeter (GE Healthcare). Titrations were performed in duplicates at 25°C with RhLNBBP (0.1 mM ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were transfected with 40 nM small interfering (si)RNA (GE Life Sciences) and 2 μL (24-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM EDTA at pH 7.4) and blotted onto Hybond-N+ membrane (GE Healthcare) using a Minifold I slot blot system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (BIO-RAD) ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... RBD:2-15 complex was then isolated by gel filtration on Superdex-200 (GE Healthcare). Fractions containing complexes were pooled and concentrated to 6.5 mg/ml in SEC buffer ...
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bioRxiv - Biochemistry 2022Quote: ... with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare) with 25 mM HEPES-KOH buffer (pH 7.5).
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bioRxiv - Cell Biology 2022Quote: A full-length zebrafish calaxin sequence was subcloned into pGEX-6P-2 vector (GE Healthcare). For the mEGFP-Calaxin protein ...
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bioRxiv - Plant Biology 2022Quote: ... for PDX3 and ACTIN-2 and captured using an Amersham Imager 680 system (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 mL of Glutathione-Sepharose resin (GE Healthcare) and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP via gel filtration (Superdex 75 Increase 10/300; GE Healthcare Life Sciences). Protein peak fractions were combined and concentrated to 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified by Ni-NTA (2 x HiTrap Chelating HP, 5 mL, GE Healthcare), buffer exchanged to cleavage buffer (20 mM TrisHCl pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 hours and then incubated with Streptavidin Sepharose High-Performance beads (GE Healthcare, 17511301) for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Immunology 2022Quote: ... a 1:1 solution of Protein A Sepharose (GE Healthcare) and Protein G Sepharose (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl Poly A (GE Healthcare #27411001, 1 mg/ml), 1 µl 10X Buffer with MgCl2 (Roche #12032902001 ...
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bioRxiv - Molecular Biology 2023Quote: ... BrdU (BU-1; RPN202 GE Healthcare Life Sciences 1:200). Secondary antibodies for immunofluorescence were as follows ...
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bioRxiv - Immunology 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) using a PD-10 desalting column (GE Healthcare, USA). Light chain of human enterokinase (10 μg/ml ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...