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Citations for GE Life Sciences :
251 - 300 of 433 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Soluble lysate was filtered through a 0.45 µm syringe filter and loaded onto a 140 mL Q Sepharose FastFlow ion-exchange column (GE Healthcare) equilibrated in buffer A (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... Pooled FluB polymerase fractions were filtered with 0.22 μm filter and loaded on a heparin column (HiTrap Heparin HP, GE Healthcare). Elution was performed with a gradient using buffers A and B (2 mM TCEP ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were dissolved in PBS pH 7.4 at 42 μM concentration and filtered with a Whatman Anotop 10 filter (20 nm cutoff, GE Healthcare) before starting the measurements ...
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bioRxiv - Microbiology 2022Quote: ... and the obtained supernatant was purified using 0.22 μm filter and passed over a Strep-Tactin II column (GE Healthcare) to collect secreted TraC ...
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bioRxiv - Cell Biology 2022Quote: ... centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then filtered through 0.2 μm filter and loaded into the Superdex 200 10/300 GL column (GE Healthcare) prewashed with balance buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was filtrated through 0.45 µm filter and loaded into HisTrap HP column (GE Healthcare, Cat#:17-5248-02). The collected fractions were concentrated by Amicon® Ultra-15 Centrifugal Filter Units (EMD Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was removed and filtered through a 0.22 μM filter and loaded onto a HisTrap High Performance column (GE Healthcare) equilibrated with buffer A containing 5 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... and 30 µL of each diluted odor was placed on a syringe filter (2.7-µm pore size, 6823-1327, GE Healthcare). Odorized air was further diluted with filtered air by 1:8 to produce a 1 liter/min total flow rate ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were filtered (0.2 µM centrifugal filter) and were then further purified by size-exclusion chromatography on Superdex 200 resin (GE Healthcare) using 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the lysate was ultracentrifuged at 22 000 g for 35 min at 4°C and the supernatant was filtered on 0.22 mm filters and then loaded on a His-Trap 5mL column (GE Healthcare) equilibrated with 50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... the supernatant containing eHEV was collected and filtered through a 0.45 um pore size filter (Whatman GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... proteoliposomes were extruded through a NucleporeTM Track-Etch Membrane polycarbonate filter with 400 nm pore size (GE Healthcare Life Siences). Reconstituted LeuT corresponding to 8 µg was treated with thrombin (0.5 unit ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and loaded onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated in binding buffer ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... The grids were blotted for 4 seconds at force 12 using double-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase ...
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bioRxiv - Molecular Biology 2022Quote: ... After passing through a 0.4 μm filter the supernatant was loaded onto a HisTrap HP 1 mL column (GE Healthcare). The protein was eluted with a lysis buffer supplemented with 300 mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and by passing through a 0.45 µm filter before loading it into a Ni-NTA column connected to an AKTA Pure instrument (GE Healthcare). After the protein binding to Ni-NTA beads ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Viral supernatant was harvested 48 h after transfection and filtered through a 0.45 μm polyethersulfone membrane filter (GE Healthcare Whatman). Western blotting of the viral supernatants with anti-V5 antibodies (below ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant passed through a 0.45 µm filter and subjected to size exclusion chromatography (SEC) using a XK 26/100 column (GE Healthcare). Fractions containing ADDoCoV were pooled and further purified by ion exchange chromatography (IEX ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting crude cell extract was filtered through a 0.45-µm filter before loading onto a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was filtered through a 0.45 µm filter and incubated with 4 ml pre-equilibrated GST beads (GE Healthcare). After 1h incubation rolling at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µM filter and loaded onto a pre-equilibrated HisTrap HP 5ml column (GE Healthcare, IL, US). The column was washed with 50 ml lysis buffer and eluted with buffer containing 200 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots of the eluate were passed through a 0.22 µm filter and injected into a HiPrep 26/60 Sephacryl S-200 column (GE Healthcare) in buffer G1 (25 mM Tris/HCl pH 8 at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was concentrated with a 30kDa cutoff filter (Amicon) and loaded onto a S200 10/300 column (GE Healthcare) equilibrated in SEC buffer (50mM Tris-HCl pH 7.5 @RT ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting protein was then concentrated using Amicon centrifugal filter units (10,000 MWCO) and further purified by size-exclusion chromatography (Superdex Hiload 75, GE Healthcare) using storage buffer (100 mM bicine ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed protein was then concentrated using Amicon centrifugal filter units (10,000 MWCO) and further purified by size-exclusion chromatography (Superdex Hiload 75, GE Healthcare) using storage buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting protein was then concentrated using Amicon centrifugal filter units (10,000 MWCO) and further purified by size-exclusion chromatography (Superdex Hiload 75, GE Healthcare) using storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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Exploring the druggability of the UEV domain of human TSG101 in search for broad-spectrum antiviralsbioRxiv - Molecular Biology 2024Quote: ... the supernatant was filtered using 0.45 µm syringe filters and loaded onto a 1 mL FPLC-GSTrap column (GE Healthcare) pre-equilibrated in Buffer A at a flow rate of 0.5 mL·min-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluate was passed through a 0.22 μm filter and applied to Sephacryl S-200 26/60 column (GE Healthcare) in SEC buffer (20 mM HEPES/KOH ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble material was subsequently filtered using a 0.22 mm pore size filter and was loaded onto a 5 ml HisTrap HP column (GE Healthcare), which was pre-equilibrated with lysis buffer and immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was filtered using a 0.45 µm filter and then loaded onto a 5 ml HisTrap™ HP nickel sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... filtered using a 0.45 µm Puradisc 25 mm polyethersulfone Syringe Filter (6780-2504, GE Healthcare Life Sciences, Whatman™, Chicago, IL) to remove cellular debris ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Water was filtered using vacuum-pumps coupled with Nalgene™ units and DNA was captured onto 0.45 μm mixed cellulose ester filters (47 mm diameter, Whatman, GE Healthcare). Generally ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the proteins were concentrated with Amicon Ultra 10K filter (viMillipore) and applied to HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions containing either AsCas12a or SpCas9 were collected ...
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant obtained at this step was filtered through 0.45 μm filters and injected into a sample loop connected to HiPrep Q FF 16/10 (GE healthcare, 28936543). The supernatant was injected at 2 mL/min and eluted using buffer B (20mM Tris-HCl ...
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bioRxiv - Bioengineering 2020Quote: ... concentrated with 100 kDa Amicon centrifugal filter units and injected into a size exclusion HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated with storage buffer G (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... the sample was filtered through a 0.22 μm filter and then purified by the Q column (GE Healthcare, 17-5156-01) and the Superdex 75 column (GE Healthcare ...