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Citations for GE Life Sciences :
2801 - 2850 of 4144 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... USA) and an Optilab T-rEX refractometer (Wyatt Technology) in-line with a Superdex 75 10/300 GL (GE Life Sciences) size exclusion chromatography analytical column ...
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bioRxiv - Biophysics 2019Quote: ... The dissolved peptide was then fractionated on a Superdex-75 HR 10/30 size exclusion chromatography (SEC) column (GE Life Sciences) on a BIORAD FPLC system that was pre-equilibrated with 20mM Tris at pH 8.00 ...
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bioRxiv - Biochemistry 2019Quote: ... and cleaved protein was concentrated and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... Fractions passed through the HisTrap HP column were collected for further purification with the Superdex 200 Increase 10/300 GL column (GE Healthcare).
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The reaction mixture was then used for purification through size-exclusion chromatography with Superdex 200 Increase 10/300 GL column (GE Healthcare) in buffer A to remove extra σ28 protein and nucleic acids.
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bioRxiv - Molecular Biology 2019Quote: ... from mouse liver was separated by 10% denaturing polyacrylamide gel electrophoresis (PAGE) and blotted onto a nylon membrane (Amersham Hybond N+; GE Healthcare) using a Transblot Turbo apparatus (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with a 50 kDa MWCO concentrator and subjected to a second SEC on a Superose 6 (10/300 GL, GE Healthcare) with a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the V2R was purified by size exclusion chromatography (SEC) using a Superdex 200 (10/300 column) connected to an AKTA purifier system (GE Healthcare). Fractions corresponding to the pure monomeric receptor were pooled (~ 2 ml ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions containing target protein was subjected to buffer exchange with buffer A using a HiPrep 26/10 desalting column (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was concentrated in a 100 kDa cut-off Vivaspin® 2 Centrifugal Concentrator to a final volume of less than 500 μl before injected onto a Superdex 200 10/300 Increase GL column (GE Healthcare), which was equilibrated with GF buffer (20 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... with a molecular weight cut-off (MWCO) of 30 kDa and polished by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: Multi-angle light scattering coupled with size exclusion chromatography (SEC-MALS) was done using a Superdex200 Increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml min−1 on an HPLC system composed of PU-2080 pumps ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were resolved by SDS-PAGE using a 10 % acrylamide gel and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Amersham Hybond, GE Healthcare) via the PerfectBlue™ tank electro blotter (Peqlab ...
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bioRxiv - Biophysics 2021Quote: ... The H2A.Z(K120C)-H2B made by combining cell pellets was also purified further by size exclusion chromatography on an S75 column (Superdex 75 Increase 10/300 GL, GE Healthcare) before storage.
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant obtained at this step was filtered through 0.45 μm filters and injected into a sample loop connected to HiPrep Q FF 16/10 (GE healthcare, 28936543). The supernatant was injected at 2 mL/min and eluted using buffer B (20mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... centrifuged at 10000g for 5min to remove precipitation and the soluble fraction was injected on a Superdex 75 10/300 (GE Healthcare). To refold EH domains ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were further purified by SEC on a Superdex 200 Increase 10/300 GL column or HiLoad Superdex 200 16/600 column (GE Healthcare). GPΔmuc-presenting NPs were produced in ExpiCHO cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... BSA was used for calibration and proteins were separated on a Superdex 200 Increase 10/300 analytical SEC column (GE Healthcare) with a flow rate of 0.4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... or 100 to 300 µg samples of individual proteins were injected onto a Superdex 75 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM TrisHCl ...
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bioRxiv - Biophysics 2021Quote: The HTT-HAP40 complex was further purified by size exclusion chromatography (SEC) using a Superose 6 10/300 increase column (GE Healthcare) in running buffer 25 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column into 20 mM NaPO4 150 mM NaCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: 150 μl sample was analyzed by Superose 6 Inc 10/300 column (Citiva, NY) equipped in ÄKTA purifier (GE healthcare, NY). The elution was conducted at 0.5 ml/min using the same buffer as used for sample at 4 °C
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted from Histrap HP column was further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with 10 mM maltose and further purified via gel filtration on a Superdex S75 (GE Healthcare) column.
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
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bioRxiv - Bioengineering 2021Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10−18 M).[20] EDTA was added to formulations (4 eq with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Unconjugated molecules were separated using size exclusion chromatohraphy with Superdex 200 Increase 10/300 GL column on ÄKTA fast protein liquid chromatography (FPLC, GE Healthcare). The purified nanosensors were concentrated by spin filters (MWCO = 10 kDa ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... protein-containing fractions were concentrated and further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). 7H8 was purified in SEC-150 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were concentrated and further purified by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Heparin buffer A containing 150 mM KCl ...
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bioRxiv - Biochemistry 2020Quote: ... Precipitate was removed by centrifugation at 13,000 rpm for 10 min and the soluble fraction was loaded onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare). Size exclusion chromatography was done in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... All recombinant forms of AnxA11 were subjected to size exclusion chromatography using a Superdex 75 or 200 Increase 10/300 GL column (GE Healthcare). Protein concentration was determined by absorbance at 280 nm (using molecular masses of 55513 ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Macrophages were cultured in complete medium: Dulbecco’s Modified Eagle Media (DMEM) supplemented with 10% fetal bovine serum (Hyclone, GE Healthcare, IL). Where indicated ...
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bioRxiv - Biochemistry 2020Quote: ... AcrB was then eluted with Buffer B containing 500 mM imidazole and directly injected onto a Superdex 75 10/600 GL size exclusion chromatography (SEC) column (GE Healthcare) equilibrated in buffer D (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... The protein eluent was concentrated and further purified by size-exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with W1 buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... 500 µL of each sample was individually injected into an S200 column (Superdex 200 10/300 GL, GE Healthcare Life Sciences) equilibrated in Buffer A and eluted with a flow rate of 0.5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: 100-500 µl samples (lipid-bound and lipid-free ApoE3 and ApoE380-255) were applied to an AKTA protein purification system (GE) equipped with a Superdex 200 10/300 (GE healthcare) column and a 500 µl sample loop and run at 0.5 ml/min at 5°C ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing CI2 determined from SDS-PAGE analysis were concentrated and loaded onto a superdex75 10/300 GL column (GE Healthcare) equilibrated with 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Following 3C protease treatment CDK2-cyclin E was buffer exchanged using SEC (Superdex 200 10/300) and then loaded onto a GST-trap column (5ml, GE Healthcare) and then onto an amylose resin (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...