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Citations for GE Life Sciences :
2751 - 2800 of 4746 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The desalted protein containing fractions were pooled and loaded on a Q-Sepharose XK16/32 column (GE Healthcare) equilibrated with 25mM MES pH 5.5 as running buffer ...
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bioRxiv - Biophysics 2020Quote: ... Protein containing fractions were loaded on SEC Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated with SEC buffer (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Cell Biology 2020Quote: ... The processed proteins were concentrated and further purified by size exclusion chromatography (Superdex-75 16/60, GE Healthcare) in running buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were further using SEC column HiLoad 16/60 Superdex 75 prep grade (GE Healthcare) previously equilibrated with 50 mM KH2PO4 ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were further purified by anion-exchange chromatography on a Resource Q column (GE Healthcare, United States) that was pre-equilibrated with buffer A (20 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the intact protein was further purified by cation exchange chromatography using a HiTrap SP HP column (GE Healthcare) and eluted by the 50– 500 mM NaCl gradient in 10 mM HEPES–KOH pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration with a Superose 6 10/300 GL column (GE Healthcare). hPCNA was expressed and purified as described in (Duffy et al ...
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bioRxiv - Biochemistry 2020Quote: All of the proteins characterized here eluted as homodimers as determined by a calibrated Superdex 75 (GE Healthcare) gel filtration chromatography column (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Protein (450 μg/100 μL) was loaded on a Superdex™ 200 HR 10/30 column (GE Healthcare) equilibrated with PBS (140 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel-filtration chromatography on a Superdex 200 16/60 column (GE Healthcare) in Cas9 storage buffer (20 mM Tris-Cl ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was subsequently loaded into an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences), pre-equilibrated in the SP buffer A to remove 6His-ZB tag and uncleaved protein ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from conditioned medium by cation exchange chromatography on a SP sepharose column (GE healthcare) in 50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system) and the images collected using a Chemidoc imaging system (BioRad Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified on a HiLoad 16/600 Superdex 75pg or 200pg gel-filtration columns (GE Healthcare). To obtain stoichiometric MLL1RWSAD complex ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (GE Healthcare Life science). Equal loading was validated using Coomassie Brilliant Blue staining of the membrane ...
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bioRxiv - Microbiology 2020Quote: ... All protein lysates were separated on 12% SDS-polyacrylamide gels and transferred to polyvinyl difluoride (PVDF, GE Healthcare) membranes ...
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bioRxiv - Plant Biology 2021Quote: ... pastoris X-33 and the secreted proteins were purified by affinity chromatography (HiTrap Chelating HP column) (GE Healthcare). Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted protein was then applied to size-exclusion chromatography (Superdex 200 Increase 10/300 GL column, GE Healthcare) with the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL Western blotting detection reagent (GE Healthcare, #GERPN2209). In a parallel gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 20X concentrated protein extract (10 ml) was loaded in an anionic-exchange Q-sepharose column (GE Healthcare, USA) previously equilibrated with 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eight days post transfection the supernatant was loaded on the HiTrap protein G HP 1ml column (GE Healthcare) in binding buffer (20 mM sodium phosphate pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was analyzed by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (GE Healthcare, #BA85). Membranes were incubated for 1 h in a blocking solution (Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was then purified by gel filtration using a Superdex 200 26/600 GL column (GE Life Sciences) before cleaving the His6-SUMO tag with ULP1 enzyme ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, cat#10600001) as previously described in [8] ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC; HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM phosphate pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were further purified by size exclusion chromatography (Superdex-75 10/300 GL column; GE Life Sciences) and judged greater than 95% pure by SDS-PAGE analysis.
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bioRxiv - Cell Biology 2022Quote: ... and 0.1 mg protein were loaded on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare) connected to an ÄKTA pure (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Biochemistry 2022Quote: ... Concentrated protein for Cryo-EM was loaded over a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) using SEC Buffer (25mM HEPES pH 7.2 ...