Labshake search
Citations for GE Life Sciences :
2751 - 2800 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... Protein containing fractions were loaded on SEC Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated with SEC buffer (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Microbiology 2019Quote: ... and O-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
-
bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using the enhanced chemiluminescence (ECL) system (ECL™ Prime Western Blotting System, GE Healthcare Bioscience).
-
bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
-
bioRxiv - Cell Biology 2019Quote: Protein purification was performed by immobilized metal-affinity chromatography (IMAC) on HisTrap HP Ni2+ Sepharose columns (GE Healthcare) at 4°C according to the manufacturer’s instructions ...
-
LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
-
bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
-
bioRxiv - Cell Biology 2020Quote: ... The processed proteins were concentrated and further purified by size exclusion chromatography (Superdex-75 16/60, GE Healthcare) in running buffer (20 mM Tris pH 7.5 ...
-
bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were further using SEC column HiLoad 16/60 Superdex 75 prep grade (GE Healthcare) previously equilibrated with 50 mM KH2PO4 ...
-
bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
-
bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant proteins were further purified by anion-exchange chromatography on a Resource Q column (GE Healthcare, United States) that was pre-equilibrated with buffer A (20 mM Tris–HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... the intact protein was further purified by cation exchange chromatography using a HiTrap SP HP column (GE Healthcare) and eluted by the 50– 500 mM NaCl gradient in 10 mM HEPES–KOH pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration with a Superose 6 10/300 GL column (GE Healthcare). hPCNA was expressed and purified as described in (Duffy et al ...
-
bioRxiv - Biochemistry 2020Quote: All of the proteins characterized here eluted as homodimers as determined by a calibrated Superdex 75 (GE Healthcare) gel filtration chromatography column (25 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: Protein (450 μg/100 μL) was loaded on a Superdex™ 200 HR 10/30 column (GE Healthcare) equilibrated with PBS (140 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel-filtration chromatography on a Superdex 200 16/60 column (GE Healthcare) in Cas9 storage buffer (20 mM Tris-Cl ...
-
bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
-
bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was subsequently loaded into an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences), pre-equilibrated in the SP buffer A to remove 6His-ZB tag and uncleaved protein ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was purified from conditioned medium by cation exchange chromatography on a SP sepharose column (GE healthcare) in 50 mM Tris ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system) and the images collected using a Chemidoc imaging system (BioRad Laboratories).
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified on a HiLoad 16/600 Superdex 75pg or 200pg gel-filtration columns (GE Healthcare). To obtain stoichiometric MLL1RWSAD complex ...
-
bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.
-
bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (GE Healthcare Life science). Equal loading was validated using Coomassie Brilliant Blue staining of the membrane ...
-
bioRxiv - Microbiology 2020Quote: ... All protein lysates were separated on 12% SDS-polyacrylamide gels and transferred to polyvinyl difluoride (PVDF, GE Healthcare) membranes ...
-
bioRxiv - Plant Biology 2021Quote: ... pastoris X-33 and the secreted proteins were purified by affinity chromatography (HiTrap Chelating HP column) (GE Healthcare). Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted protein was then applied to size-exclusion chromatography (Superdex 200 Increase 10/300 GL column, GE Healthcare) with the buffer containing 25 mM MES pH 6.0 ...
-
bioRxiv - Microbiology 2021Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL Western blotting detection reagent (GE Healthcare, #GERPN2209). In a parallel gel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
-
bioRxiv - Plant Biology 2021Quote: ... 20X concentrated protein extract (10 ml) was loaded in an anionic-exchange Q-sepharose column (GE Healthcare, USA) previously equilibrated with 20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... Eight days post transfection the supernatant was loaded on the HiTrap protein G HP 1ml column (GE Healthcare) in binding buffer (20 mM sodium phosphate pH 7.0 ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was analyzed by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (GE Healthcare, #BA85). Membranes were incubated for 1 h in a blocking solution (Tris-buffered saline (TBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein was then purified by gel filtration using a Superdex 200 26/600 GL column (GE Life Sciences) before cleaving the His6-SUMO tag with ULP1 enzyme ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, cat#10600001) as previously described in [8] ...
-
bioRxiv - Cell Biology 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC; HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM phosphate pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... the proteins were further purified by size exclusion chromatography (Superdex-75 10/300 GL column; GE Life Sciences) and judged greater than 95% pure by SDS-PAGE analysis.
-
bioRxiv - Cell Biology 2022Quote: ... and 0.1 mg protein were loaded on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare) connected to an ÄKTA pure (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
-
bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
-
bioRxiv - Biochemistry 2022Quote: ... Concentrated protein for Cryo-EM was loaded over a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) using SEC Buffer (25mM HEPES pH 7.2 ...
-
bioRxiv - Biochemistry 2022Quote: ... Then the protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...