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Citations for GE Life Sciences :
2651 - 2700 of 4746 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The cleaved proteins were further purified by cation exchange chromatography using HiTrap SP HP column (GE Healthcare) equilibrated in buffer C ...
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bioRxiv - Microbiology 2024Quote: ... The mAbs were isolated from the culture supernatants by affinity chromatography using Protein G beads (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted proteins were loaded onto a HiTrap Q HP 5 ml anion exchange column (GE Healthcare) pre-equilibrated with QA buffer ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified using a GE Healthcare HisTrap HP nickel column (GE HealthCare, Chicago, US). Purified proteins were dialyzed at 4 °C for 24 hours using a MW14000 dialysis membrane (Biosharp ...
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bioRxiv - Neuroscience 2024Quote: ... Immune complexes were incubated with Protein G Sepharose® 4 Fast Flow (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were finally purified by passing through the HiLoad 16/600 Superdex 75 column (GE Healthcare). The nsp16/nsp10 complex was eluted as a single homogenous species in a final buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... proteins were separated according to molecular weight by SDS-PAGE using the Ettan DaltTwelve system (GE Healthcare). Radioactive gels were dried on Whatman paper and exposed to storage phosphor screens (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... 100 μg of APOs200 proteins were resolved in a Superdex Increase 200 10/300 GL (GE Healthcare) on an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... Dialysed protein was further purified by ion-exchange chromatography using a HiTrap-FF Q column (GE Healthcare), resulting in the elution of N-myc proteins in the flow-through and at low salt concentrations ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the protein was purified using Ni-NTA affinity columns on an ÄKTA pure system (GE healthcare), followed by size exclusion chromatography in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The one-step purified protein was then loaded onto a HiTrap Heparin Sepharose HP column (GE Healthcare) and eluted from it in a salt gradient of 100-1000 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted on a nickel affinity column (HisTrap HP, 1mL, GE Healthcare, 17-5247-01) using an ÄKTA Pure 25M Chromatography System (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified and buffer exchanged using the Superdex 300 Increase 10/300 GL (GE Healthcare) size exclusion column into the lysis buffer for storage and characterization ...
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bioRxiv - Biochemistry 2024Quote: ... The purified proteins were taken for the size exclusion chromatography column (Superdex 75G 16/600, GE Healthcare) using the AKTA Prime purification system (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from these gels were transferred onto a previously activated PVDF membrane (Hybond-P, GE Healthcare Ltd) in an electro-blotting apparatus (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). The membranes were then probed with the following antibodies ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-PAGE and transferred to a Protran nitrocellulose membrane (GE Healthcare Life Sciences) using a wet transfer apparatus (Amersham Bioscience) ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and immediately loaded onto a size-exclusion column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... and then add peroxidase substrate solution to detect expression of the protein of interest (GE Healthcare Amersham).
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit or antirat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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bioRxiv - Cancer Biology 2021Quote: ... incubated with appropriate HRP-labelled secondary antibodies (GE Healthcare Life Sciences), washed as before and developed using ECL (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody was detected using the ECL plus reagents (GE Healthcare). Detection was done in a dark room ...
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bioRxiv - Systems Biology 2021Quote: ... primary antibodies and polyclonal goat anti-mouse HRP conjugated (GE Healthcare) secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... Secondary HRP-conjugated antibodies used were donkey anti-rabbit (GE Healthcare) and goat anti-mouse (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used was anti-rabbit HRP-conjugated from GE Healthcare.
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bioRxiv - Cancer Biology 2020Quote: ... and HRP-conjugated secondary antibodies: anti-rabbit (GE Healthcare Cat# NA934); anti-mouse (GE Healthcare Cat# NA931 ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-linked monkey anti-rabbit secondary antibody was from GE Healthcare Life Sciences (Little Chalfont ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibody was anti-rabbit IgG (GE Healthcare, 1:30000). Signal detection was performed with SuperSignal™ West Femto Maximum Sensitivity Substrate chemiluminescence detection system (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated anti-mouse-IgG antibody (GE Healthcare, Chicago, IL, USA), diluted 1:5,000 in blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... the HRP-linked anti-rabbit IgG secondary antibody (GE Healthcare, NA934) was used at a dilution of 1:5000 or 1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... and HRP-linked donkey anti-mouse IgG antibody (NA931V; GE Healthcare). Cell supernatants containing the pseudotype viral particles were centrifuged at 109,000 g or 35 min at 4 °C using a TLA100.3 rotor with an Optima TLX ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-coupled antibodies were detected by enhanced chemiluminescence (Amersham, GE Healthcare) and X-ray films ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Phages were detected by HRP-conjugated anti-M13 antibody (GE Healthcare). For signal generation ...
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bioRxiv - Biochemistry 2020Quote: ... Antibody complexes were detected using enhanced chemiluminescence detection reagents (GE Healthcare) and digital images captured using GeneSnap (SynGene ...
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bioRxiv - Immunology 2020Quote: ... Purification of labelled antibodies was performed using NAP5 Columns (GE HealthCare). The dye to antibody ratio was quantified using Nanodrop and only antibodies with a composition of 3-4 Alexa Fluor 405 and 0.9-1.2 Alexa Fluor 647 per antibody were used for imaging.
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bioRxiv - Immunology 2021Quote: ... Hit detected by fluorescent secondary antibody using ImageQuant software (GE Healthcare). An expression vector (plRES-hEGFR- IRES-ZsGreen1 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were analyzed by size exclusion chromatography (SEC) (Cytiva, GE Healthcare) and separated for monomeric fraction in case aggregation levels were >5% ...
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bioRxiv - Genetics 2020Quote: ... the membranes were incubated with HRP-conjugated secondary antibodies (GE Healthcare) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies and signal was detected using ECL Plus (GE Healthcare). Blots were stripped and re-probed with anti-actin antibody (Santa Cruz).
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bioRxiv - Cancer Biology 2022Quote: ... Bound antibodies were detected using chemiluminescence (ECL, GE Healthcare, Cat# GERPN2232) and imaged using the Fusion Fx Imaging System (Vilber ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-rabbit and anti-mouse IgG-HRP-conjugated antibodies (GE Healthcare) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... horseradish peroxidase–linked anti-rabbit and anti-mouse antibodies (GE Healthcare) were used at 1:10,000 dilutions ...
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with ECL Plus Detection substrate (GE Healthcare) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... secondary α-mouse-HRP conjugated antibody (GE Healthcare, Life Sciences NA931) was used ...